Regulated Environment Variant Confirmation
PCR + Sanger sequencing allows you to detect or confirm mutations in genomic DNA. This technique is a reliable method for initial analysis or orthogonal confirmation of next generation sequencing, microarrays, or qPCR/dPCR results in clinical samples.
Performed in our state-of-the-art CLIA-certified and CAP-accredited laboratory, and processed by trained personnel on qualified equipment, this service includes optional QA oversight, variant report and/or lab director signature. This service is not CLIA-validated and is not intended for clinical use. The data is research use only.
Service Details
| Service Level* | Raw Data Files | QA Oversight | Variant Report | Lab Director Signature | Test Registration | Clinical Outcome Report |
|---|---|---|---|---|---|---|
| Regulated Environment |
Included | Available as an add-on | Not Available
All service levels are performed by trained personnel in a CLIA-certified lab with qualified equipment.
Applications
SAMPLE TO ANSWER
1. PROJECT CONSULTATION & PRIMER DESIGN
Amplicon selection and primer design to targeted regions of interest in new or existing assays
2. ASSAY DEVELOPMENT
Optimization on project-specific samples with different validation options
3. PCR & PURIFICATION
PCR amplification of genomic DNA using ideal conditions
4. SEQUENCING & DATA ANALYSIS
Bi-directional DNA sequencing per amplicon with optional variant reporting
Deliverables
All customers receive raw data as .ab1, .seq, and .phd files. QA oversight, variant report and lab director signed quality score report are available as an add-on.
