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Sample Submission Guidelines

Sanger Sequencing

Sanger Sequencing is a cost-effective method for determining the nucleotide sequence of DNA. GENEWIZ Sanger sequencing services are award-winning, providing high-quality results, industry-leading customer service and fast turnaround times at competitive prices. GENEWIZ from Azenta Life Sciences is the partner of choice for academic, pharmaceutical, biotechnology, and government institutions around the world.

We are well-equipped to handle DNA sequencing of all complexities including single tube, high-throughput, direct bacterial colony, or glycerol stock DNA sequencing projects. Our mission is to eliminate bottlenecks, improve productivity, and advance your research progress.

GENEWIZ Sanger Sequencing Service

GENEWIZ can process a variety of different templates to use as starting material for Sanger sequencing reactions submitted in tubes or plates.

Click on the service below to view the service options available.

Pre-Mixed or Pre-Defined Sanger Sequencing Services and Barcodes

GENEWIZ offers two service types for Sanger sequencing of PCR products or plasmids: Pre-Mixed or Pre-Defined, both available for tubes and plates. Our barcode-driven automated workflows enable flawless and convenient sample processing. Barcode labels can easily be ordered and shared with colleagues and managed through the Payment Center within our GENEWIZ ordering system and sample submitted at your nearest dropbox or via a local courier.

Prepaid Barcodes: Secure flexibility, convenient logistics, automated sample processing workflows, and easy online order tracking throughout the entire process. Enjoy minimized paperwork and simplified invoicing, boosting your sequencing efficiency.
Non-Prepaid Barcodes: Use these with any of our sequencing services for a flexible selection of your specific needs. Activate Non-Prepaid Barcodes in your CLIMS account to choose and utilize the desired sequencing service.

Labelling Your Samples

▼Pre-Mixed and Pre-Defined Tubes

Pre-Mixed Tube

For sequencing plasmids or purified PCR products, this service is the most economical choice.
You provide your template and primer mixed in the same tube according to our Sample Submission Guidelines.

Pre-Defined Tube

You submit your template and primer in separate tubes, and a GENEWIZ scientist will mix them when your sample is received. An additional charge applies in most cases.
For sequencing plasmids, purified PCR products, or un-purified PCR products submit your template and primer in separate tubes according to our Sample Submission Guidelines.
You can also select your primer from our list of GENEWIZ Universal Primers or synthesize your custom primer to be stored in our lab.

Pre-Mixed or Pre-Defined Tube Barcode Colors

Premixed or Pre-Defined Tubes Standard Delivery	Premixed or Pre-Defined Tubes Express Delivery
Data will be ready for download the next business day after sample receipt at GENEWIZ. Place Order	Data will be ready for download the next business day by 12 pm after sample drop off at your GENEWIZ dropbox. Place Order

▼Pre-Mixed and Pre-Defined Plates

Pre-Mixed Plate (Full Plate Service)

Suitable for purified templates (purified plasmids or purified PCR products), this service is available for high-throughput Sanger sequencing of 96 samples.
For this service we will always invoice 96 reactions (= 1 plate) even if less than 96 samples are submitted.
You provide your template and primer mixed in the same 96-well plate according to our Sample Submission Guidelines.

Pre-Defined Plate (Full Plate Service)

Suitable for plasmids, purified PCR products, un-purified PCR products or bacterial colonies/glycerol stocks this service is available for high-throughput Sanger sequencing of 96 samples.
For this service we will always invoice 96 reactions (= 1 plate) even if less than 96 samples are submitted.
1 or more primer can be submitted to sequence the entire plate (with each primer)
You provide your templates/samples in a 96-well plate and your sequencing primer in a separate tube according to our Sample Submission Guidelines.
You can also select your primer from our list of GENEWIZ Universal Primers or synthesize your custom primer to be stored in our lab.
Express delivery (data provided on the next business day after sample Dropoff to our Dropboxes) for Sanger sequencing Plates (full plates) only available for pre-mixed samples.
Data delivery for pre-Defined samples submitted in full-plates will be done with our Standard priority (data available on the next business day after sample receipt)

Pre-Mixed Vario Plate

Suitable for purified templates (purified plasmids or purified PCR products), this service is available for high-throughput Sanger sequencing of 96 sample or less samples.
For this service, we will only invoice the number of reactions that are submitted/requested on a corresponding Vario plate.
You provide your template and primer mixed in the same well of a 96-well plate according to our Sample Submission Guidelines.

Pre-Defined Vario Plate

Suitable for purified templates plasmids, purified PCR products, un-purified PCR products or bacterial colonies/glycerol stocks this service is available for high-throughput Sanger sequencing of 96 sample or less samples.
For this service, we will only invoice the number of reactions that are submitted/requested on a corresponding Vario plate.
Primers can be submitted and assigned to your sequencing reactions in a flexible manner (up to 4 different primers per well).
You provide your templates/samples in a 96-well plate and your sequencing primer in a separate tube according to our Sample Submission Guidelines.
You can also select your primer from our list of GENEWIZ Universal Primers or synthesize your custom primer to be stored in our lab.
Express delivery (data provided on the next business day after sample Dropoff to our Dropboxes) for Sanger sequencing Vario plates (full plates) is available for pre-mixed samples and pre-defined already purified samples (purified plasmids or PCR products).

Pre-Mixed or Pre-Defined Plates Barcode Color

Premixed or Pre-Defined Plates Standard Delivery	Premixed or Pre-Defined Plates Express Delivery
Data will be ready for download the next business day after sample receipt at GENEWIZ. Place Order	Data will be ready for download the next business day by 12 pm after sample drop off at your GENEWIZ dropbox. Place Order

▼Primers

Custom or GENEWIZ Primers

If you wish to use a custom primer, use purple Primer Barcodes to label your primer tube for the Pre-Defined Tube or Pre-Defined Plate/Vario Plate Sequencing Service.
If you want to use a free GENEWIZ Universal Primer or would like us to synthesize a primer for you, please define the Primer you want to use for your sequencing reaction when you place your Sanger sequencing order on your CLIMS account.

Custom Primer Barcode Color

Custom Primers
For our pre-defined tube or pre-defined Pplate Sequencing services only Place Order

▼Primer Walking

Primer Walking Services

Utilizes multiple primers in a series to sequence entire plasmids or PCR products
Service options include plasmid sequence confirmation for already known sequences and plasmid sequence discovery for unknown sequences
For AAV vectors, we offer primer walking plus our new, proprietary AAV-ITR Sequencing

Plasmid Sequence Confirmation Service	Plasmid Sequence Discovery Service
Confirm the sequence of your cloned products/genes of interest Sequence your entire plasmid, including difficult-to-amplify regions Examine failed amplifications and incorrect restriction digest patterns Sequence full length AAV plasmids and hard to sequence ITR regions	Create a plasmid map for your undetermined plasmids Confirm the sequence of your mystery constructs Identify unknown regions of your plasmids Sequence full length AAV plasmids and hard to sequence ITR regions

Sanger Sequencing Features & Benefits

High Quality – DNA sequencing read lengths up to ~1,100 nt. Quality Score and Contiguous Read Length (CRL) provided in each read.

GENEWIZ Technical Support – Our highly-trained team of Sanger sequencing specialists proactively communicate updates, while providing technical expertise on projects big and small.

Clever, Convenient Logistics – Contact us to locate a GENEWIZ dropbox near you for free shipping. GENEWIZ will provide you with suitable logistical resources according to your barcode order and priority choice.

Easy Online Ordering – Simply order sequencing barcodes online in CLIMS or activate and assign available prepaid barcodes while placing your online order in your CLIMS account.

Barcodes for Sanger Sequencing – Provides fully-automated workflows, unambiguous sample assignment, online order tracking and secured data retrieval from your online CLIMS account.

Free GENEWIZ Universal Primers – a wide selection of Universal Primers are available for standard vectors. Use our Universal Primer Selection Tool to determine which Universal Primer binds to your vector of choice.

Quick Turnaround Times

Standard

Data reported next business day after samples are received at GENEWIZ.
Express

Data reported by 12 pm next business day after sample drop off at your nearest GENEWIZ dropbox*

* Terms and Conditions apply to turnaround times. For UK customers, regional variations in turnaround times also apply. Please speak to your Sales Representative for more information.

Technical Resources

Sanger Sequencing Quick Tips Guide

Learn from the Sanger experts how you can get the most of your DNA Sequencing. Whether you’re troubleshooting a tough sequencing reaction, perfecting PCR visualization, or learning to clean up and refine your PCR products, these resources are designed to help you get the best results.
• Volume 1: Producing Robust, Single-band PCR Product
• Volume 2: PCR Clean-up of Single-band PCR
• Volume 3: Salvaging Nonspecific PCR Products
• Volume 4: PCR Visualization by Gel Electrophoresis
• Volume 5: Troubleshooting a Bad Sequencing Reaction from a PCR Template

Blog | Troubleshooting DNA Templates with Sanger Sequencing

Sanger sequencing is highly effective for testing small targeted genomic regions and for validating results from NGS. However, as with all sequencing technologies, there are limitations and needs for troubleshooting. Read on to learn about the three basic troubleshooting steps we recommend to start with.

Blog | Ensure Complete Plasmid Validation on Your First Attempt with Oxford Nanopore Sequencing

Explore the cutting-edge realm of genomic research with Oxford Nanopore Sequencing (ONT), and how this innovative Next Generation Sequencing technology offers real-time, long-read capabilities, revolutionizing our approach to decoding genetic information.

Webinar | From Base Pairs to Breakthroughs: Understanding Sanger and Oxford Nanopore Sequencing

While Sanger sequencing remains the gold standard for accurate DNA sequencing, advancements in sequencing technology, such as Oxford Nanopore Technology (ONT), have provided a cost-effective, long-read alternative for analysis. In this workshop, delve into the considerations of each technique and discover how to interpret their resulting sequencing data effectively so you can capture the benefits of both approaches for your specific needs.

Blog | Analyzing Sanger Sequencing Data

The output for Sanger sequencing is typically a chromatogram, also known as a trace or ab1 file, and a text-based sequence file. Although the latter may seem to hold all the relevant information—after all, the point of sequencing is to get a sequence—the former can’t be ignored. Here, we provide a guide to understanding Sanger sequencing data.