Regulated Environment Sanger Sequencing
This standalone Sanger sequencing service utilizes sample processing in a regulated environment to support assays performed on clinical samples. Performed in our state-of-the-art CLIA-certified and CAP-accredited laboratory, and processed by trained personnel on qualified equipment, this service includes optional QA oversight and/or lab director signature. This service is not CLIA-validated and is not intended for clinical use. The data is research use only.
Regulated environment Sanger sequencing is best utilized as the initial readout from a PCR assay or in conjunction with next generation sequencing, specifically to elucidate complex regions not approachable by other methodologies or to confirm variants uncovered by genome-wide techniques.
Service Details
| Service Level* | Raw Data Files | QA Oversight | Variant Report | Lab Director Signature | Test Registration | Clinical Outcome Report |
|---|---|---|---|---|---|---|
| Regulated Environment |
Included | Available as an add-on | Not Available
All service levels are performed by trained personnel in a CLIA-certified lab with qualified equipment.
Features And Benefits
Regulated Environment Sanger Sequencing Service Options
Pre-Mixed
For sequencing plasmids or purified PCR products, provide your template and primer mixed in the same tube for the most economical CLIA-compliant services.
Pre-Defined
If choosing our Pre-Defined Service, submit your plasmids or purified PCR products and primer in separate tubes, and a GENEWIZ scientist will mix them once your sample arrives on site.
Custom
Full-service option allows you to submit unpurified PCR product with unknown concentration along with your primer for sequencing. We will purify your PCR product as well as measure and adjust the concentration to optimize your reactions.
Template Types
Purified Plasmids or PCR Products
Un-Purified PCR Products
Difficult Template Sequencing
Deliverables
All customers will receive their raw data as .ab1, .seq, and .phd files along with a quality score report.