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NGS-based amplicon sequencing allows detection of low frequency variants, quantitative analysis of mixed populations, and scalable analysis of a large number of samples. Whether you are looking to validate/discover variations in specific genomic regions or are interested in screening clones, GENEWIZ offers multiple options for ultra-deep sequencing of amplicons (PCR products). GENEWIZ employs the latest technologies to provide custom, in-depth solutions for amplicons ranging from 100 base pairs (bp) to >10,000 bp.
Applications for this service include antibody library screening, natural antibody repertoire profiling, disease genotyping, CRISPR mutation analysis, 16/18S gene sequencing, screening of molecular clones, and analysis of plasmid stocks.
NGS Amplicon Services
Multiple Solutions to Answer Every Biological Question
| GENEWIZ's Recommendation | ||||
| Amplicon Size | Applications | Library Preparation Type | Platform Configuration | |
| 100-250 bp |
|
Adapter ligation to |
Illumina 2x150 bp | |
| 250-550 bp |
|
Adapter ligation to full-length products |
Illumina 2x250 bp or 2x300 bp |
|
|
Fragmentation | Illumina 2x150 bp | ||
| >550 bp |
|
Adapter ligation to full-length products |
PacBio Sequel | |
|
Fragmentation | Illumina 2x150 bp | ||
Features & Benefits
Sample Submission Guidelines
GENEWIZ accepts purified and unpurified amplicons, restriction digested material, fragmented DNA, and plasmids. For detailed sample submission requirements please visit our Sample Submission Guidelines.
