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Next Generation Sequencing
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Sequencing OnlyRNA-SeqSingle-Cell RNA SequencingExome SequencingAmplicon Sequencing ServicesWhole Genome SequencingCRISPR ValidationTargeted SequencingMetagenomics SolutionsEpigenomicsImmunogenomics
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Sanger Sequencing
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Service & OptionsBarcodes for Sanger SequencingLogistic Options
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Gene Synthesis
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Standard Gene Synthesis (PriorityGENE)TurboGENE™FragmentGENESynthetic DNA LibrariesCRISPR Construct SynthesisssDNA Synthesis NewDNA CloningSite-Directed Mutagenesis
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PCR + Sanger Services
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Bacterial and Fungal IdentificationMutation AnalysisSNP GenotypingPCR Plus Sequencing CRISPR Analysis PackagecDNA & gDNA Verification ServicesYeast Colony SequencingFragment Analysis
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Plasmid DNA Preparation
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Standard Plasmid DNA PreparationTargeted Yield Plasmid DNA Preparation
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Oligo Synthesis Services
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Oligo-Rapid SynthesisOligo-Flex SynthesisOligo Retained for Sanger Sequencing
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GLP-Compliant Services
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Confirmatory SequencingSNP Mutation Analysis
Plasmid Prep
Nucleic Acid Extraction
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Clinical Services NEW
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CLIA Variant Confirmation (PCR + Sanger)CLIA Sanger SequencingCLIA Whole Exome SequencingCLIA Whole Genome Sequencing
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AMPLICON SEQUENCING SERVICES
NGS-based amplicon sequencing allows detection of low frequency variants, quantitative analysis of mixed populations, and scalable analysis of a large number of samples. Whether you are looking to validate/discover variations in specific genomic regions or are interested in screening clones, GENEWIZ offers multiple options for ultra-deep sequencing of amplicons (PCR products). GENEWIZ employs the latest technologies to provide custom, in-depth solutions for amplicons ranging from 100 base pairs (bp) to >10,000 bp.
Applications for this service include antibody library screening, natural antibody repertoire profiling, disease genotyping, CRISPR mutation analysis, 16/18S gene sequencing, screening of molecular clones, and analysis of plasmid stocks.
NGS Amplicon Services
Multiple Solutions to Answer Every Biological Question
| GENEWIZ's Recommendation | ||||
| Amplicon Size | Applications | Library Preparation Type | Platform Configuration | |
| 100-250 bp |
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Adapter ligation to |
Illumina 2x150 bp | |
| 250-550 bp |
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Adapter ligation to full-length products |
Illumina 2x250 bp or 2x300 bp |
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Fragmentation | Illumina 2x150 bp | ||
| >550 bp |
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Adapter ligation to full-length products |
PacBio Sequel | |
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Fragmentation | Illumina 2x150 bp | ||
Features & Benefits
Sample Submission Guidelines
GENEWIZ accepts purified and unpurified amplicons, restriction digested material, fragmented DNA, and plasmids. For detailed sample submission requirements please visit our Sample Submission Guidelines.
