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NGS-based amplicon sequencing allows detection of low frequency variants, quantitative analysis of mixed populations, and scalable analysis of a large number of samples. Whether you are looking to validate/discover variations in specific genomic regions or are interested in screening clones, Azenta Life Sciences offers multiple options for ultra-deep sequencing of amplicons (PCR products). Azenta employs the latest technologies to provide custom, in-depth solutions for amplicons ranging from 100 base pairs (bp) to >10,000 bp.
Applications for this service include antibody library screening, natural antibody repertoire profiling, disease genotyping, CRISPR mutation analysis, 16/18S gene sequencing, screening of molecular clones, and analysis of plasmid stocks.
NGS Amplicon Services
Multiple Solutions to Answer Every Biological Question
| Azenta's Recommendation | ||||
| Amplicon Size | Applications | Library Preparation Type | Platform Configuration | |
| 100-250 bp |
|
Adapter ligation to |
Illumina 2x150 bp | |
| 250-550 bp |
|
Adapter ligation to full-length products |
Illumina 2x250 bp or 2x300 bp |
|
|
Fragmentation | Illumina 2x150 bp | ||
| >550 bp |
|
Adapter ligation to full-length products |
PacBio Sequel | |
|
Fragmentation | Illumina 2x150 bp | ||
Features & Benefits
Technical Resources
Blog | NGS, qPCR, or Sanger Sequencing: An Assay Selection Guide
This selection guide offers practical information about PCR + Sanger, qPCR, and NGS approaches to help you determine which assay best suits your project requirements, along with an interactive assay selection tool to aid your decision making.
Sample Submission Guidelines
Azenta accepts purified and unpurified amplicons, restriction digested material, fragmented DNA, and plasmids. For detailed sample submission requirements please visit our Sample Submission Guidelines.
