Next Generation Sequencing

Sample submission guidelines

DNA Sample Submission Guidelines

DNA can be concentrated free of charge via SpeedVacTM. Additional charges will apply for concentration via column or other methods.

Whole Genome Sequencing

  • Sample Type: Genomic DNA
  • Sample Purity (OD260/280): 1.8-2.0
  • Recommended Quantity: >1.5 µg, >20 ng/µl
  • Minimum Amount: 500 ng
  • Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
  • Shipment Method: Dry Ice

ChIP-Seq Sample Submission Guidelines

  • Sample Type: ChIP DNA
  • Sample Purity (OD 260/280): 1.8-2.0
  • Recommended Quantity: >50 ng
  • Minimum Amount: 10 ng
  • DNA Fragment Length: 100-800 bp; 200-300 bp (Main peak)
  • Resuspension Buffer: Water, BE, or low TE (<0.1 mM EDTA)
  • Shipment Method: Dry Ice

Agilent SureSelect Exome Enrichment Kit, OncoGxOne™ Discovery Cancer Panels and TargetGxOne™ Gene Enrichment

  • Sample Type: Genomic DNA
  • Sample Purity (OD260/280): 1.8-2.0
  • Recommended Quantity: >1.5 µg, >20 ng/µl
  • Minimum Amount: 500 ng
  • Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
  • Shipment Method: Dry Ice

Custom TargetGxOne™ Amplicon Sequencing

  • Sample Type: Genomic DNA
  • Sample Purity (OD260/280): 1.8-2.0
  • Recommended Quantity: >1 µg, >20 ng/µl
  • Minimum Amount: 100 ng
  • Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
  • Shipment Method: Dry Ice

16S MetaVx™ Panels

  • Sample Type: Genomic DNA
  • Sample Purity (OD260/280): 1.8-2.0
  • Recommended Quantity: >1 µg, >20 ng/µl
  • Minimum Amount: 100 ng
  • Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
  • Shipment Method: Dry Ice

Pre-made Sequencing Libraries

  • Recommended Quantity: >15 nM
  • Volume: ≥20 ul
  • Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
  • Shipment Method: Dry Ice
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RNA Sample Submission Guidelines

Standard/Strand-Specific RNA-Seq

  • Sample Type: Total RNA
  • Sample Purity (OD260/OD280): 1.8-2.2
  • Recommended RIN: ≥6.0
  • Recommended Quantity: >2 µg, >50 ng/µl
  • Minimum Quantity: 500 ng
  • Resuspension Buffer: Nuclease-free water
  • Shipment Method: Dry Ice

Small RNA-Seq

  • Sample Type: Total RNA or Purified Small RNA
  • Sample Purity (OD260/OD280): 1.8-2.2
  • Recommended RIN: ≥6.0
  • Recommended Quantity: Total RNA: >2 µg, >50 ng/µl, Purified Small RNA: >50 ng, >5 ng/µl
  • Resuspension Buffer: Nuclease-free water
  • Shipment Method: Dry Ice

Targeted RNA-Seq

  • Sample Type: Total RNA
  • Sample Purity (OD260/OD280): 1.8-2.2
  • Recommended RIN: ≥6.0
  • Recommended Quantity: >2 µg, >50 ng/µl
  • Minimum Quantity: 1 µg
  • Resuspension Buffer: Nuclease-free water
  • Shipment Method: Dry Ice

Ultra-Low Input RNA-Seq

Cell number and buffer clearly indicated in GENEWIZ Sample Submission Form

  • Sample Type: Cells in solution or small amounts of total RNA
  • Recommended Quantity: >1,000 cells/sample in Trizol or 20 ng of total RNA
  • Shipment Method: Dry Ice

Single-Cell RNA-Seq

  • Sample Type: Frozen cells according to GENEWIZ-supplied protocol, submitted in duplicate
  • Sample Viability: >90% post-thaw
  • Recommended Quantity: >1M cells in 1 mL
  • Minimum Quantity: 25K cells in 500 µL
  • Resuspension Buffer: Appropriate cryopreservation media (e.g., cell culture media supplemented with 20% FBS and 10% DMSO)
  • Shipment Method: Dry Ice overnight
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Extraction Requirements

All extractions are performed to best of GENEWIZ’s ability and the Client is charged for work performed regardless of outcome. Additional charges for procurement of kits may apply for select extractions.

FFPE Samples

  • Recommended Quantity: ≥4 FFPE slides recommended
  • Minimum Quantity: 2 FFPE slides
  • Thickness: 5µm-20 µm
  • Diameter: >150 mm2
  • Shipping Method: Room Temperature/Blue Ice

Cell pellets

  • Recommended Quantity: 106 cells
  • Minimum Quantity: 104 cells
  • Shipping Method: Dry Ice

Fresh Frozen Tissue

  • Recommended Quantity: 10 mg
  • Minimum Quantity: 2 mg
  • Shipping Method: Dry Ice

Viral Particles

  • Recommended Quantity: >1 mL supernatant
  • Viral Titer: >5x109 cells/mL
  • Shipping Method: Dry Ice

Whole Blood

  • Recommended Quantity: 2 tubes/sample
  • Recommended Volume (DNA Extraction): 500 µl/tube
  • Minimum Volume (DNA Extraction): 200 µl/tube
  • Recommended Volume (RNA Extraction): 2.5 mL (whole blood, PAXgene Blood RNA Tube)
  • Shipping Method: Fresh: Blue Ice, Frozen: Dry Ice

Plasma/Serum

  • Recommended Quantity: 2 tubes/sample
  • Recommended Volume: 10 mL/tube
  • Minimum Volume: 5 mL/tube
  • Shipping Method: Dry Ice

Stool

  • Recommended Quantity: 100 mg
  • Minimum Quantity: 50 mg
  • Shipping Method: Dry Ice/Blue Ice

Saliva

  • Recommended Quantity: 1 mL
  • Shipping Method: Dry Ice/Blue Ice

Soil

  • Recommended Quantity: 100 mg
  • Minimum Quantity: 50 mg
  • Shipping Method: Room Temperature/Blue Ice

Water

  • Recommended Volume: 100 mL
  • Minimum Volume: 20 mL
  • Shipping Method: Room Temperature

Swab

  • Recommended Quantity: 2 tubes/sample. 1 swap/tube
  • Shipping Method: Room Temperature

Fixed Needle Core Biopsy

  • Recommended Quantity: 4 biopsies
  • Minimum Quantity: 1 biopsy
  • Recommended Diameter: 1-2 mm diameter; >5 mm in length
  • Shipping Method: Room Temperature/Blue Ice

Snap Frozen Needle Core Biopsy

  • Recommended Quantity: 10 mg
  • Minimum Quantity: 3 mg
  • Shipping Method: Dry Ice
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 GENEWIZ Sample Quality Control Assessment

GENEWIZ performs QC free of charge with results reported within 24 hours.

  DNA RNA Pre-Made Libraries
Agilent 2100 BioAnalyzer  

Qubit Assay


Gel Electrophoresis
   

For questions on submitting other, or less than ideal sample types, please contact our Project Management team to discuss further as we may be able to proceed with your project.

Phone: +1 (908) 222-0711 ext. 1

Email: NGS@genewiz.com

 

Direct Shipping (FedEx, UPS): 

Please use an overnight delivery service such as FedEx or UPS for all NGS samples. A print out of the confirmed quote must be included with the material.


All direct shipments should be sent to:

  
GENEWIZ 
Attn: Next Generation Sequencing 
115 Corporate Boulevard 
South Plainfield, NJ 07080 
USA 
908-222-0711