PLASMID DNA PREPARATION
Delivering high quality DNA from a variety of starting materials in a cost-effective and reliable manner is our priority. Don’t waste your valuable time with a plasmid preparation kit when GENEWIZ Plasmid Preparation Services can conveniently provide plasmid DNA preps at the scale and quantity you need.
Our standard plasmid DNA preparation service is a flexible and fast choice for a range of plasmid templates. Runs are available in the classic mini to giga scales, yielding micrograms to milligrams of purified plasmid DNA. Additional add-on service and analysis options are available, enabling project customization to meet budgetary and technical specifications.
Features & Benefits
Standard Plasmid Prep Service Options
| Prep Service | Turnaround Time | Plasmids Accepted | Spectrophotometric DNA Quality Analysis | Transfection-grade (Endotoxin Free) | DNA Normalization | GLP-Compliant | Certificate of Analysis |
|---|---|---|---|---|---|---|---|
Standard-Scale (Per Run) |
Starting from 2-3 business days |
|
Included | Add-On | Add-On | Add-On | Add-On |
Standard Plasmid Prep Analysis Options
| Prep Service | DNA Sequencing* | Full-Service DNA Sequence Confirmation | Restriction Digest and Agarose Gel Analysis* | Sterility Testing | DNA Homogeneity % Supercoiled DNA Quantification | Endotoxin Level Quantification |
|---|---|---|---|---|---|---|
Standard-Scale (Per Run) |
Add-On | Add-On | Add-On | Add-On | Add-On | Add-On |
*Includes one reaction per plasmid; added reactions available as an add-on.
DNA PREPARATION SCALES AND YIELDS
| Run Scale | Typical Yield for High-Copy, Stable Plasmids^ | Typical Yield for Low-Copy, Unstable, or Toxic Plasmids^ |
| Midi | 70-100 μg | 10-50 μg |
| Maxi | 350-500 μg | 50-250 μg |
| Mega | 1.5-2.5 mg | 250 μg-1.25 mg |
| Giga | 7-10 mg | 1-5 mg |
| Mini Preps | Typical Yield for High-Copy, Stable Plasmids^ | Typical Yield for Low-Copy, Unstable, or Toxic Plasmids^ |
| Tube | 7-10 μg | 2-5 μg |
| 96-well Plates | 5-10 μg | 1-3 μg |
^Actual yield will vary with the nature of the plasmid, bacterial hosts, and other factors. Please contact gs@azenta.com to discuss known low-copy, unstable, or toxic constructs.
Choice of Starting Material
DNA
Transformation provided as a service*
Glycerol Stock
Provide 200 μL frozen stock
Bacterial Colony
Please provide fresh colonies (<3 days old)
*For DNA samples, GENEWIZ provides multiple host strains to choose from for transformation. For custom strains, please provide the starting material as bacterial colony or clonal glycerol stock, or select “Other” for host strain when placing your order and include the competent cells with your DNA submission.
Tech Note | Optimizing Plasmid Preparation to Increase Yield and Reduce Endotoxins
Plasmid DNA (pDNA) is vital for a range of biopharmaceutical and biotechnological applications but maximizing yield while minimizing endotoxins can be challenging. This tech note explores strategies to optimize bacterial growth conditions, enhance lysis, and refine purification protocols to boost pDNA yield and purity, ensuring consistent, high-quality results.