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PLASMID DNA PREPARATION
GENEWIZTM Plasmid Preparation Services from Azenta provide high-quality plasmids from microgram- to gram-scale with reliable and fast turnaround times to accommodate tight deadlines. Speed, flexibility, and quality of DNA obtained are critical factors for any plasmid DNA prep service.
Whether ordering standard plasmid preps for cloning, transformation, or in vitro experiments, or transfection-grade plasmid preps for cell culture and other endotoxin-sensitive applications, GENEWIZ is the vendor of choice.
Plasmid DNA Preparation Services
Standard (Per-Run) Plasmid Preparation
A per-run plasmid preparation service with order flexibility and the fastest starting turnaround time. Includes standard yield and purity testing, with additional service and analysis options available. Ideal for all plasmids, whether high-yield and stable or low-yield and toxic constructs.
Targeted Yield Plasmid Preparation
An amount-based bundled plasmid preparation solution with popular service and analysis options built-in. Includes free upgrade to transfection-grade DNA, sequencing and restriction digest analysis, and sterility verification. Ideal for high-copy, stable plasmids.
GLP Plasmid Preparation
GLP-compliant, endotoxin- and animal-free plasmid preparations are available and performed in compliance with FDA and EPA GLP regulations. This approach is ideal for cell-line development projects supporting FDA submissions and as internal controls for biomarker assays and kits. Animal-free plasmid preparations are also available for R&D efforts.
AAV Plasmid Preparation
Our proprietary AAV plasmid preparation service provides superior quality from midi- to giga-scale AAV vectors. This service provides includes pre-and post-preparation quality control (QC) steps that leverage our AAV-ITR Sanger sequencing capabilities for full sequence confirmation, and to ensure delivery of clones containing intact ITR regions. This service is ideal service for cell and gene therapy research utilizing recombinant AAV DNA.
= Included
= Add-on
= Not Available
| Standard (Per-Run) | Targeted Yield (Amount Based) | ||
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Service Options |
Turnaround Time | Starting from 2-3 business days | Starting from 4-5 business days |
| Plasmids Accepted |
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| Spectrophotometric DNA Quality Analysis | |||
| Transfection-grade (Endotoxin Free) | |||
| DNA Normalization | |||
| GLP-Compliant | |||
| Analysis Options | DNA Sequencing* | ||
| Full-Service DNA Sequence Confirmation | |||
| Restriction Digest and Agarose Gel Analysis* | |||
| Sterility testing |
*Includes one reaction per plasmid; added reactions available as an add-on
Choice of Starting Material
DNA
Transformation provided as a service*
Glycerol Stock
Provide 200 μL frozen stock
Bacterial Colony
Please provide fresh colonies (<3 days old)
*For DNA samples, GENEWIZ provides multiple host strains to choose from for transformation. For custom strains, please provide the starting material as bacterial colony or clonal glycerol stock, or select “Other” for host strain when placing your order and include the competent cells with your DNA submission.
HOW TO ORDER
direct-sequencing templates are not available for our Same Day service.
