Maximize Resources with Plasmid Preparation Services
High-Quality Plasmid DNA Preparation to Meet Tight Deadlines
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Plasmid Prep Service
GENEWIZ Plasmid Preparation Services provide high-quality plasmids from microgram- to gram-scale with reliable and fast turnaround times to accommodate tight deadlines. Speed, flexibility, and quality of DNA obtained are critical factors for any plasmid DNA prep service.
Whether ordering standard plasmid preps for cloning, transformation, or in vitro experiments, or transfection-grade plasmid preps for cell culture and other endotoxin-sensitive applications, GENEWIZ is the vendor of choice.
General Plasmid DNA Preparation Services
Standard-Scale Plasmid Preparation
A per run plasmid preparation service with order flexibility and the fastest starting turnaround time. Includes standard yield and purity testing, with additional service and analysis options available. Ideal for all plasmids, whether high-yield and stable or low-yield and toxic constructs.
Targeted Yield Plasmid Preparation
An amount-based bundled plasmid preparation solution with popular service and analysis options built-in. Includes free upgrade to transfection-grade DNA, sequencing and restriction digest analysis, and sterility verification. Ideal for high-copy, stable plasmids. Now offering large-scale, high-quality plasmids ranging from 250 µg to 5 grams. Ideal for cell and gene therapy, drug discovery, and vaccine research.
Service & Analysis Options
= Included
= Add-on
= Not Available
| Standard-Scale (Per Run) | Targeted Yield (Amount Based) | ||
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Service Options |
Turnaround Time | Starting from 3-4 business days | Starting from 4-5 business days |
| Plasmids Accepted |
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| Spectrophotometric DNA Quality Analysis | |||
| Transfection-grade (Endotoxin Free) | |||
| DNA Normalization | |||
| Analysis Options | DNA Sequencing* | ||
| Full-Service DNA Sequence Confirmation | |||
| Restriction Digest and Agarose Gel Analysis* | |||
| Sterility Testing |
*Includes one reaction per plasmid; added reactions available as an add-on.
FEATURES & BENEFITS
*100 mg to 5 g services is processed in operation sites outside Europe.
Choice of Starting Material
DNA
Transformation provided as a service*
Glycerol Stock
Provide 200 μL frozen stock
Bacterial Colony
Please provide fresh colonies (<2 days old)
*For DNA samples, GENEWIZ provides multiple host strains to choose from for transformation. For custom strains, please provide the starting material as bacterial colony or clonal glycerol stock, or select “Other” for host strain when placing your order and include the competent cells with your DNA submission.
Specialized Plasmid DNA Preparation Services
GLP Plasmid Preparation
GLP-compliant, endotoxin- and animal-free plasmid preparations are available and performed in compliance with FDA and EPA GLP regulations. This approach is ideal for cell-line development projects supporting FDA submissions and as internal controls for biomarker assays and kits. Animal-free plasmid preparations are also available for R&D efforts.
AAV Plasmid Preparation
Our proprietary AAV plasmid preparation service provides superior quality from midi- to giga-scale AAV vectors. This service provides includes pre-and post-preparation quality control (QC) steps that leverage our AAV-ITR Sanger sequencing capabilities for full sequence confirmation, and to ensure delivery of clones containing intact ITR regions. This service is ideal service for cell and gene therapy research utilizing recombinant AAV DNA.
Tech Note | Optimizing Plasmid Preparation to Increase Yield and Reduce Endotoxins
Plasmid DNA (pDNA) is vital for a range of biopharmaceutical and biotechnological applications but maximizing yield while minimizing endotoxins can be challenging. This tech note explores strategies to optimize bacterial growth conditions, enhance lysis, and refine purification protocols to boost pDNA yield and purity, ensuring consistent, high-quality results.
HOW TO ORDER
direct-sequencing templates are not available for our Same Day service.
