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Highly Scalable
Submit one to hundreds of samples
Flexible Data Amounts
Starting at 10M paired end reads
Latest Platforms
Illumina® NovaSeq™ delivers the highest throughput
European Provider
Sequencing available at our lab in Leipzig, Germany
RNA SEQUENCING
RNA sequencing (RNA-Seq) is a powerful method for studying the transcriptome qualitatively and quantitatively. It can identify the full catalog of transcripts, precisely define the structure of genes, and accurately measure gene expression levels.
Azenta Life Sciences provides unparalleled flexibility in the analysis of different RNA species (coding, non-coding, and small transcripts) from a wide range of starting material using long- or short-read sequencing.
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WORKBOOK
Exploring Bioinformatics for Genomic and Transcriptomic Sequencing Data
A gateway into the world of bioinformatics
- Discover what tools you'll need for
bioinformatics analysis - Explore helpful resources to perform more in-depth exploration
- Follow step-by-step instructions on how to analyze data
Download Now
RNA Sequencing Services
Single-Cell RNA-Seq
Single-cell RNA sequencing analyzes gene expression at single-cell resolution for heterogeneous samples. The 10x Genomics® Chromium™ platform provides advanced transcriptional profiling of thousands of individual cells.
Standard RNA-Seq
Standard RNA sequencing is our most popular option for profiling gene expression, enabling the analysis of coding (mRNA) and long non-coding RNA (lncRNA).
CLIA RNA-Seq
RNA-Seq services performed in a CAP/CLIA laboratory for clinical applications. Custom CLIA validations for specific assays.
Find the right NGS solution for your project using our interactive guide.
Features & Benefits
Superior data quality with a guarantee of ≥80% bases with ≥Q30 for 2x150 bp sequencing (>90% on average)
Industry-leading turnaround time as fast as 1 week
Dedicated support from Ph.D.-level project managers at every step including post-delivery support
Complete sample-to-analysis solutions including flexible extraction and data analysis options available
Technical Resources
Technical Specifications
Example Data
Sample Submission Guidelines
Frequently Asked Questions
GENEWIZ Citations
Tech Note | Full-Length RNA-Seq: A Novel Method to Assess Sequence Integrity for RNA Therapeutics
Strict quality control is required to maintain the integrity of manufactured products for RNA therapies, but current assays often present limitations. Learn how the novel full-length RNA-Seq approach from Azenta allows you to preserve the entire length of and effectively sequence the poly(A) tails of your mRNA products.
Workbook | Exploring Bioinformatics for Genome and Transcriptome Sequencing Data
For those new to bioinformatics, analyzing massive amounts of NGS data can be a daunting task. Download Azenta’s bioinformatics workbook to learn how to analyze whole genome sequencing (WGS) and RNA sequencing (RNA-Seq) data with bioinformatics tools to reveal biological insights for your research.
Webinar| Non-Classical Sources of Tumour-Specific Antigen in Checkpoint Inhibitor Response
Developing immunotherapies for cancer can be difficult due to the variation of immune response from patient to patient. In this webinar, Dr. Litchfield from UCL Cancer Institute presents his team’s exploratory research using a multiomics approach to better understand the diversity of immune response to cancer and highlights their findings of an alternative source of a tumour-specific antigen in checkpoint inhibitor (CPI) response.
Webinar Series | Advancing Transcriptomics: Gene Expression Screening, Single-Cell RNA-Seq, and Beyond
With this two-part webinar series, go beyond traditional transcriptomics and learn about the various NGS approaches available for gene expression analysis. In part 1, we take an in-depth look at various gene expression approaches, including RNA-Seq, single-cell RNA-Seq, digital spatial profiling, and more. In part 2, we explore the data generated from these approaches and how they can complement each other and confirm findings.
RNA-Seq Bioinformatics Workshop & Roundtable Discussion
RNA-Seq bioinformatics can be complex and difficult to decipher. To help make it more approachable, this workshop and roundtable discussion, led by Azenta Life Sciences' bioinformatics manager Brian Sereni, explores the bioinformatics pipeline, explains NGS results, and addresses common challenges and FAQs for RNA-Seq bioinformatics analysis.
Tech Note: Add More Life to Your Data: Optimizing Single-Cell RNA-Seq with Dead Cell Removal
Obtaining samples with high cell viability can be difficult for many experiments but is necessary for success on the 10x Genomics® Chromium™ platform. This tech note describes how Azenta scientists used optimized single-cell workflows, including dead cell removal, to overcome low viability and generate high-quality sequencing data.
Case Study: Uncovering Cell Type-Specific Expression Profiles in the Tumor Microenvironment with Ultra-Low Input RNA-Seq
Biomedical specimens are often restricted to minute quantities, posing major limitations to RNA-Seq. This case study shows how approximately 50 sorted cells from a glioblastoma can produce transcriptomic data comparable to RNA-Seq experiments that use millions of cells.
Case Study: Single-Cell RNA-Seq Analysis Identifies Rare Drug-Resistant Cancer Stem Cells
Cell populations are rarely homogeneous and synchronized in their characteristics. Standard RNA-Seq approaches are limited to reporting general expression levels thus omitting minor subpopulation profiles. This study highlights new single-cell RNA sequencing capabilities for identifying rare cells, characterizing their transcriptomes, and discovering potential biomarkers.
Tech Note: Isoform Sequencing on the PacBio Sequel® – Maximizing Output and Accuracy
Contiguous mRNA full-length sequencing (Iso-Seq) greatly simplifies genome annotation efforts and revolutionizes the discovery of novel RNA isoforms. This Tech Note discusses the advantages of the latest technologies combined with Azenta’s optimized workflow, and how this increases output and accuracy.
Webinar: Towards High Dimensional Sequencing: From Single-Cell to Spatial Omics
In comparison to traditional profiling methods which assess bulk populations, single-cell technologies empower researchers to examine diversity of heterogeneous cell populations and uncover new, and potentially unexpected, biological discoveries. This webinar highlights the unparalleled capabilities of single-cell sequencing.
Tech Note: Achieving Phenotypic Profiling for Rapid Drug Discovery with High-Throughput Gene Expression Screening
High-throughput technologies are critical in performing phenotypic profiling for drug discovery applications. In this tech note, Azenta Life Sciences discusses the challenges associated with traditional approaches, such as microarrays and RNA sequencing, and offers an optimized assay to achieve high-quality phenotypic profiling at a reduced cost for rapid drug discovery.
Blog | NGS, qPCR, or Sanger Sequencing: An Assay Selection Guide
This selection guide offers practical information about PCR + Sanger, qPCR, and NGS approaches to help you determine which assay best suits your project requirements, along with an interactive assay selection tool to aid your decision making.
Blog | Fine-Tuning mRNA Structure for Better mRNA Therapies
The structure of mRNA has been known for decades, but only in recent years have researchers unlocked its potential for therapeutic development. With the right delivery vehicle, mRNA products can replace defective proteins in the cell, generate antigens for immunization (e.g., COVID vaccines), or edit the genome via CRISPR technology. Let’s review the structural features of a functional mRNA molecule and discuss how to optimize these for therapeutic applications.
NGS PLATFORMS
ILLUMINA® NOVASEQ™/HISEQ®
PACBIO® SEQUEL®
10X GENOMICS® CHROMIUM™
