RNA SEQUENCING

 

RNA sequencing (RNA-Seq) is a powerful method for studying the transcriptome qualitatively and quantitatively. It can identify the full catalog of transcripts, precisely define the structure of genes, and accurately measure gene expression levels.

GENEWIZ provides unparalleled flexibility in the analysis of different RNA species (coding, non-coding, and small transcripts) from a wide range of starting material using long- or short-read sequencing.

 

RNA Sequencing Services

Single-Cell RNA-Seq

Single-cell RNA sequencing analyzes gene expression at single-cell resolution for heterogeneous samples. The 10x Genomics® Chromium™ platform provides advanced transcriptional profiling of thousands of individual cells.

Ultra-Low Input RNA-Seq

Ultra-low input RNA sequencing provides bulk expression analysis of samples containing as few as 10 pg of RNA or just a few cells. Recommended for sequencing cells after fluorescence-activated cell sorting (FACS).

Iso-Seq

Iso-Seq (isoform sequencing) reads full-length contiguous transcripts. Applications include genome annotation, gene fusion detection, novel transcript discovery, and alternative splicing analysis.


Standard RNA-Seq

Standard RNA sequencing is our most popular option for profiling gene expression, enabling the analysis of coding (mRNA) and long non-coding RNA (lncRNA).

Strand-Specific RNA-Seq

Strand-specific RNA sequencing provides gene expression information with relation to strand orientation. Used for genome annotation, analysis of anti-sense/overlapping transcripts, and novel transcript discovery.

Small RNA-Seq

Small RNA sequencing enables analysis of miRNA and other small RNA species. Provides small RNA discovery and profiling.

Features & Benefits

Highly Scalable

Submit one to hundreds of samples

Flexible Data Amounts

Starting at 10M paired end reads

Latest Platforms

Illumina® NovaSeq™ delivers the highest throughput

European Provider

Sequencing available at our lab in Leipzig, Germany


Superior data quality with a guarantee of ≥80% bases with ≥Q30 for 2x150 bp sequencing (>90% on average)

Industry-leading turnaround time as fast as 1 week

Dedicated support from Ph.D.-level project managers at every step including post-delivery support

Complete sample-to-analysis solutions including flexible extraction and data analysis options available

Tech Note: Add More Life to Your Data: Optimizing Single-Cell RNA-Seq with Dead Cell Removal

Obtaining samples with high cell viability can be difficult for many experiments but is necessary for success on the 10x Genomics® Chromium™ platform. This tech note describes how GENEWIZ scientists used optimized single-cell workflows, including dead cell removal, to overcome low viability and generate high-quality sequencing data.

Case Study: Uncovering Cell Type-Specific Expression Profiles in the Tumor Microenvironment with Ultra-Low Input RNA-Seq

Biomedical specimens are often restricted to minute quantities, posing major limitations to RNA-Seq. This case study shows how approximately 50 sorted cells from a glioblastoma can produce transcriptomic data comparable to RNA-Seq experiments that use millions of cells.

Case Study: Single-Cell RNA-Seq Analysis Identifies Rare Drug-Resistant Cancer Stem Cells

Cell populations are rarely homogeneous and synchronized in their characteristics. Standard RNA-Seq approaches are limited to reporting general expression levels thus omitting minor subpopulation profiles. This study highlights new single-cell RNA sequencing capabilities for identifying rare cells, characterizing their transcriptomes, and discovering potential biomarkers.

Tech Note: Isoform Sequencing on the PacBio Sequel® – Maximizing Output and Accuracy

Contiguous mRNA full-length sequencing (Iso-Seq) greatly simplifies genome annotation efforts and revolutionizes the discovery of novel RNA isoforms. This Tech Note discusses the advantages of the latest technologies combined with GENEWIZ’s optimized workflow, and how this increases output and accuracy.

NGS PLATFORMS

ILLUMINA® NOVASEQ™/HISEQ®

PACBIO® SEQUEL®

10X GENOMICS® CHROMIUM™