To support mRNA research and therapeutic applications, Azenta offers gene synthesis and custom mRNA synthesis with industry-leading project completion time, a wide range of deliverables, and best-in-class quality.
Synthetic mRNA comes from a template nucleotide sequence of DNA. The process starts with codon optimization of this sequence for optimal transcription. The gene is synthesized, cloned into a vector, and verified by Sanger sequencing. The plasmid DNA is then amplified, target mRNA sequence linearized, and used as a template for in vitro transcription.
With the critical role of mRNA vaccines in global pandemic response to COVID-19, mRNA synthesis technology has strengthened its status as an important technique in therapeutic development. Sequence accuracy of the template plasmid and the quality of IVT mRNAs are two crucial factors in determining the success of downstream lead generation and characterization. A foundational expertise in gene synthesis accommodates a range of mRNA complexities (5’ cap and poly A tail) to achieve optimal protein expression and generate 100% sequence-verified plasmid constructs. Download the tech note to learn how Azenta achieves high-quality analytical and empirically performing mRNA products.
mRNA products are provided as a dry powder, fully lyophilized and shipped at room temperature. Below is a summary of what to expect from your custom mRNA synthesis order.
|mRNA Synthetic Motifs||Purified Synthetic Yield||Synthetic Length
||Estimated Completion Time||Quality Control|
|ncRNA: Non-coding RNA
Cap0 mRNA: m7Gppp-capped mRNA with polyA tail
Cap1 mRNA: m7GpppNm-capped mRNA with polyA tail
Pseudouridine modification (optional)1
40 ug – 2000 ug
|< 2000 bp||In as few as
|- 100% sequence accuracy from Sanger sequencing of template
- Certificate of analysis (CoA)
- Gel electrophoresis size visualization
- Agilent 2100 Bioanalyzer verification (optional)2
|2001-4000 bp||In as few as
|4001-6000 bp||In as few as
> 6001 bp
If you have custom requests or require any pre-project consultation, please contact our expert project management team.
Poly(A) tail sequences can impact the integrity of your mRNA plasmids for in vitro transcription. While longer poly(A) tails have been shown to increase their stability, tails greater than 100 bases are susceptible to truncations. Learn how the proprietary mRNA plasmid preparation protocol from Azenta can help you generate higher yields and preserve poly(A) tails of greater lengths compared to standard protocols for high-fidelity templates in our tech note.