Gene Synthesis Services
Because Discovery Can’t Wait
Synthesis Without Limits
GENEWIZ Gene Synthesis services deliver fast, reliable custom gene synthesis with 100% sequence verification, and turnaround times as fast as 5 business days. Our new, upgraded ordering platform makes it easy to design your construct, clone into a GENEWIZ vector or any custom vector, view transparent pricing, and order in minutes, with optional high-quality plasmid preparation available for downstream applications.
The GENEWIZ DIFFERENCE
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Any Insert. Any Cloning Method.
Synthesize the most complex sequences, with any cloning method and vector of your choice.
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Trusted Quality for Over 25 Years
>99.8% project success rate with >95% on-time delivery
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Support at Every Step
Ph.D.-level scientists provide real-time technical consultation and project updates across 10+ time zones.
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Free Custom Vector Onboarding
Easily onboard your vectors through our dropbox network, with 2-year free storage.
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Over
120,000Scientists Served Across 8,000+ Institutions
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Trusted by
30/30Top Pharmaceutical Companies
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Supporting Discoveries in
37+Nobel Laureate Labs
Introducing Gene Synthesis 2.0
Speed You Need. Quality You Trust.
| Service Options | Sequence Length | Turnaround Time* | Sequence Complexity | Vectors** | Quality Control |
|---|---|---|---|---|---|
 SPRINT |
Up to 3 kb | 5-6 Business Days | Simple Sequences Only | Standard Vectors | Sanger Sequencing |
 FLEX |
Up to 150+ kb | As Fast as 7 Business Days | All Sequences | Any Vectors | Sanger Sequencing + Restriction Enzyme Analysis |
 FLEX+ |
Up to 150+ kb | As Fast as 5 Business Days | All Sequences | Any Vectors | Sanger Sequencing + Restriction Enzyme Analysis |
2–5 µg (Bundle Plasmid Prep for Higher Yield)
*Turnaround time reflects production time only; additional time may be required for shipping.
**Choose from the GENEWIZ vector library or onboard your own vector for free. SPRINT: Available for simple backbones only (vector restrictions apply).
See available vectors
| Cloning Vectors | Expression Vectors – Mammalian | Expression Vectors – Bacterial | Expression Vectors – Insect |
|---|---|---|---|
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pUC-GW-Kan pUC-GW-Amp pBluescript II KS(+) pBluescript II SK(+) |
pGW-cDNA 3.1 pGW-cDNA 3.1 GFP pGW-cDNA 3.1 mCherry pGW-cDNA 3.1 DYK pGW-cDNA 3.1 HA pGW-cDNA 3.4 |
pET-11a pET-15b pET-21a pET-22b pET-24a pET-28a pET-30a pET-32a |
pGW-Bac1 pGW-BacDual |
Upgraded AI/ML-Powered Codon Optimization Engine for Higher Expression
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The upgraded GENEWIZ codon optimization tool combines our proven heuristic framework with advanced AI/ML trained on high-expression datasets to deliver higher, more reliable codon-driven gains across sequences
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Built into our upgraded ordering experience
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Supports 20+ expression hosts
New Upgraded Online Ordering
Build Your Own Order in Under 3 Minutes
Why Researchers Choose GENEWIZ
“The entire process was very smooth and easy, I am very satisfied with the new interface and all the options. The new options, including DNA prep scale choices, bulk codon optimization, species selection for optimization, offering the GENEWIZ version of pcDNA3.4, etc., are much appreciated. The cheaper cost is also greatly appreciated. Really excited to keep using this tool!”
“Overall, it worked very well. I had a very positive experience. Compared to other portals I have used, this is very user-friendly. Cost is very reasonable, excellent with small genes. I really like the part where I can add a custom vector so easily.”
Technical Resources
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eBook | A Guide to AAV in Gene Therapy
AAV vectors play a critical role in gene therapy, offering an ideal approach for targeted gene delivery. However, understanding the complexities of AAV packaging, optimization, and quality control testing can be challenging. In this eBook learn step-by-step strategies for mastering AAV vector development and enhancing your gene therapy applications.
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Tech Note | Enhancing Protein Expression by Leveraging Codon Optimization
Codon preference is among the most critical parameters affecting the expression level of foreign genes. This tech note will discuss two practical case studies, comparing wild-type gene performance to our codon-optimized sequences.
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Guide | Competent Cell Selection
This guide will help you choose protocols for making competent cells and appropriate strains for your experiment.
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Application Note | De Novo Synthesis of a 150 kb Construct
Building and manipulating large DNA molecules pose several complications that are problematic for de novo synthesis and sequence verification. In this application note, we discuss how our established difficult template protocols enable accurate construction of even the most challenging regions