WHOLE GENOME SEQUENCING

 

Receive the comprehensive genome analysis you need for your research project. Whole genome sequencing (WGS) provides unprecedented access to genomic information — expediting breakthroughs in healthcare, oncology, biomarker discovery, agriculture, and metagenomics.

GENEWIZ’s expertise in the latest sequencing technologies and bioinformatics solutions, combined with long-read sequencing on the PacBio Sequel system, enable us to deliver high-quality data and analysis for the genomes of all organisms including humans, animals, plants, bacteria, and viruses. 

CLIA-compliant whole genome sequencing (WGS) is also available for clinical research.

Service Package

Whole Genome Sequencing service package includes:

 

Service Options

 Starting Material   Prior to library preparation
 Deliverables

 Double-stranded gDNA

 —

 

Human:
•Quality Report, Raw data FASTQ files, SNP/INDEL detection, structural variation, and CNV analysis.
•Tumor/normal analysis

Non-Human:
•Standard DNA analysis 
•CNV discovery
De novo genome assembly
•Whole genome metagenomics analysis





Other Sources*



gDNA extraction

Table 1: Starting material that can be accepted for Whole Genome Sequencing services at GENEWIZ
 

*A wide range of starting materials can be accepted: FFPE samples, fresh frozen tissue, cell pellets etc. View our full list and Sample Submission Guidelines

 

Features and Benefits

Starting from one sample
Automated library preparation
Flexible packages for human and non-human samples
Superior data quality
Industry-leading turnaround time
Real time project updates
Comprehensive suite of bioinformatics solutions available 
Dedicated Ph.D.-level support team at every project step 

Workflow

  • Sample Submission
    1
  • Automated library preparation
    2
  • Sequencing using Illumina latest technology
    3
  • Raw data FASTQ files
    4

APPLICATIONS

De Novo Assembly

Assemble novel genomes without a reference sequence

Variant Detection

Identify SNVs, short INDELs, and germline/somatic mutations

Structural Variants

Discover >50 bp INDELs, translocations, inversions, and copy number variations

Haplotype Phasing

Determine which alleles are co-located on the same chromosome

Need additional samples for your cohort study?

Get streamlined access to Nashville Bio's world-class biobank

Technical Resources

Case Study | Detection of Previously Unidentified Structural Variants Involved in Glioblastoma Using PacBio® Sequel® Sequencing​

This case study looks at how GENEWIZ utilized the PacBio Sequel long-read technology to help a customer improve detection of structural variants within glioblastoma samples.​

Tech Note | Multiplexing Microbes on the PacBio Sequel: Feasibility, Benefits, and Recent Developments​

This study proves the feasibility of multiplexing and highlights the key advantages of the PacBio Sequel platform as compared to its predecessor, the RSII.​

Best Practices | Preparation of High Molecular Weight DNA​

High-quality high molecular weight (HMW) genomic DNA (≥50 kb) is critical to achieving long read lengths on platforms such as the 10x Genomics® Chromium™ and the PacBio Sequel. Follow these guidelines to generate the best possible WGS results.​

NGS PLATFORMS

SEQUEL® SYSTEM

  • Long-read technology delivers up to 60 kb reads with contig N50 >1 Mb​
  • Best sensitivity for structural variant calling

NOVASEQ® | HISEQ® | MISEQ®

  • Maximum throughput with lowest cost per base​
  • Single-cell whole genome sequencing available​

CHROMIUM™ CONTROLLER

  • Chromosomal characterization through linked-read sequencing
  • Haplotype phasing in ~10 Mb blocks

Get access to GENEWIZ's global world-class NGS service capabilities