Azenta Life Sciences© NGS solutions can be leveraged for adeno-associated virus (AAV) applications, providing a streamlined approach for AAV cell & gene therapy research. Combined with our proprietary QC process, these solutions enable researchers to verify the integrity of packaged AAV vectors, including inverted terminal repeat (ITR) regions, measure AAV expression after infection, monitor host response, and much more.

Whether you are looking for AAV plasmid sequencing or interested in post-infection solutions, Azenta utilizes the latest technologies on the market, including PacBio® long-read (>10kb) and Illumina® short-read sequencing, to provide custom, in-depth solutions for complex AAV gene therapy applications.


Confirm Plasmid Sequences

Utilize Azenta's© short-read amplicon sequencing for highly-accurate confirmation of AAV plasmid inserts, or confirm full-length recombinant AAV (rAAV) vector integrity at the single-molecule level with long-read amplicon sequencing.

Validate Packaged Material

Sequence-confirm rAAV vectors and identify DNA contaminants in AAV library preparation by employing the power of Azenta's© short- and long-read whole genome sequencing solutions.

Quantify AAV Expression

Employ Azenta's© qPCR, targeted sequencing, or RNA sequencing solutions at the RUO and CLIA levels to measure AAV expression of the target gene after infection.

Monitor Host Response

Stratify and characterize host-immune response during and after infection with Azenta's© suite of RNA sequencing solutions, or use novel approaches including single-cell sequencing and spatial genomics.

Learn more about Azenta's© additional adeno-associated virus (AAV) services for your cell and gene therapy research.


  • 1. Sample

  • 2. Library

  • 3. Sequencing

  • 4. Bioinformatics


  • Superior Data Quality
    Exceeding manufacturer’s benchmarks

  • Real-Time Project Updates
    Through our online system

  • Ph.D.-Level Support
    At every step

  • Trusted partner supporting over 150 top global pharmaceutical and biotech customers in their cell and gene therapy research

  • Optimized, automated, and scalable workflows with stringent quality control producing high-quality, consistent results

  • RUO and CLIA-grade, high-capacity and high-throughput sequencing for projects of virtually any size and sample type

  • Customized and extensive bioinformatics solutions with interactive, hands-on analysis


    Adeno-associated Virus Genome Population Sequencing Achieves Full Vector Genome Resolution and Reveals Human-Vector Chimeras

    Discover how researchers utilized single molecule, real-time (SMRT®) sequencing to profile rAAV-packaged genomes and assess full-length integrity for rAAV quality control.

    Advanced Characterization of DNA Molecules in rAAV Vector Preparations by Single-stranded Virus Next-generation Sequencing

    In this study, learn how short-read sequencing technology can extensively characterize the rAAV genome while simultaneously detecting the presence of DNA contaminants.

    Novel Sequencing & Synthetic Solutions for AAV-Based Gene Therapy Research

    Adeno-associated virus (AAV) vectors are an optimal vehicle for gene therapy delivery, but can present challenges for researchers. In this interview-style webinar first aired at the PacBio Global Virtual Summit 2020, Azenta presents proprietary sequencing and synthesis solutions to overcome the obstacles presented by AAV-based gene therapy.

    A Comprehensive Guide to Using AAV Vectors in Gene Therapy

    Working with AAV vectors can be challenging. In this on-demand workshop & roundtable discussion, you’ll gain a better understanding of the AAV development pipeline for gene therapy research and learn how to optimize upstream and downstream processes including AAV synthesis, sequencing, bioinformatics, and storage.


Azenta is a certified service provider for Illumina and other cutting-edge NGS platforms. For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. Azenta does not guarantee data output or quality for sequencing only projects.

How To Order

Email | Phone +49 (0)341 520 122-41