CRISPR VALIDATION

Recent advancements in genome editing techniques, namely CRISPR/Cas9, have greatly facilitated cell line engineering, gene targeting, and the development of transgenic models. However, identification of correctly targeted cells remains a significant bottleneck for large-scale projects.

To remove this hurdle, GENEWIZ developed genoTYPER-NEXT™, a proprietary NGS-based assay ideal for ultra-sensitive, high-throughput, sample-to-answer genotyping projects. This tool is more effective and efficient than T7E1, TIDE, and IDAA assays for screening targeted cell lines.

We also offer targeted, whole genome, and amplicon sequencing NGS solutions to aid on- and off-target analysis of genome editing.

 

  
 

 

Improved Genome Editing Using Single-Stranded DNA




 

genoTYPER-NEXT: HIGH-THROUGHPUT ASSAY FOR CRISPR VALIDATION

1. SUBMIT SAMPLES

Submit CRISPR-edited cell lines in 96-well plates​

2. AMPLIFY ON/OFF-TARGET SITE(S)

Cells lysis and PCR with barcoded primers​

3. SEQUENCE​

Samples pooled and sequenced on Illumina® platform​

4. ANALYZE DATA INTERACTIVELY​

Visualize results in genoTYPER-NEXT browser​

View our other NGS services for CRISPR Validation

FEATURES & BENEFITS

Complete sample-to-answer solution starting from cells to genotype (explore our interactive report; log in required)
Automated, high-throughput workflow – Up to 10,000 samples per run​
Hassle-free - Avoid gDNA extraction, pre-screening prior to sequencing, and TA cloning​
Highly sensitive – Detection of <1% allele frequency, full INDEL resolution, and frameshift analysis
More reasons to choose GENEWIZ

Case Study | Rapid, Scalable, and Ultra-Sensitive Validation of Allele-Specific Gene Editing Events with genoTYPER-NEXT™​

Discover the powerful capabilities of genoTYPER-NEXT in this illuminating case study and learn how this high-throughput assay allows rapid and accurate genotyping of CRISPR-edited cell lines.​

Webinar | Rapid and Sensitive Validation of Gene Editing with Novel NGS Assays​​

Learn about current techniques to validate gene edits and how recent advancements in next generation sequencing are improving the sensitivity and scalability of screening genomic manipulations.​

Get access to GENEWIZ's global world-class NGS service capabilities