WHOLE PLASMID SEQUENCING: PLASMID-EZ

 

Plasmid-EZ is an end-to-end solution that enables cost-effective, rapid, and high-throughput sequencing of whole plasmids. With conventional methods, only important regions of plasmids are sequenced which can fail to detect unwanted changes in other areas during replication and leave you to piece together an entire plasmid map. Using long-read sequencing, Plasmid-EZ provides complete verification of plasmid integrity and fidelity.

Now connected to our expansive dropbox network, Plasmid-EZ offers unparalleled convenience for sample submission. Simply order directly through our customer portal and deposit your samples at one of our convenient dropboxes.

 

 

What is whole plasmid sequencing?

Whole plasmid sequencing is the comprehensive read and analysis of a plasmid’s entire genetic code, known as plasmid DNA (pDNA).

Whole plasmid sequencing provides detailed insights into the genetic makeup of plasmids and how subsequent modifications of plasmids can be used in gene knockout and editing studies. By sequencing the whole plasmid, genomic data can be used to further the understanding of microbial diversity, evolution, and mechanisms underlying biological processes.

How is whole plasmid sequencing done?

Whole plasmid sequencing is carried out by isolating the plasmid DNA (pDNA), splicing the pDNA into smaller fragments, sequencing said fragments, then assembling the plasmid sequence data to reconstruct the full plasmid genome. Bioinformatics tools are then used to analyze the resulting sequencing data.

PLASMID CONSTRUCTION WORKFLOW WITH PLASMID-EZ

 



COMPLETE PLASMID SEQUENCING WORKFLOW FOR PLASMID VALIDATION

 



Technology Comparison: Whole Plasmid Sequencing

Metric Sanger Sequencing Plasmid-EZ Primer Walking NGS
Starting Material Plasmid DNA Plasmid DNA Plasmid DNA Plasmid DNA
Region Sequenced Targeted Regions Full-Length Plasmid Long Targeted Regions Full-Length Plasmid
Cost $ $$ $$$ $$$
Read Quality +++ ++ +++ ++++
Read Length ++ ++++ ++ +/++++
Automatic Assembly
Gene Annotations
Other Requirements Must provide primer Must provide reference

Deliverables

• FASTQ files
• Read-length and quality report
• Consensus plasmid assembly
• Plasmid annotation files

 

Confirm your plasmid sequences with Azenta, a worldwide leader in sequencing since 1999

 

 

Features & Benefits

 
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Quick Turnaround Times

For rapid, complete plasmid validation

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Easily Submit Samples

At any one of our convenient dropbox locations

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Interactive Data Report

Provides an easy way to understand sequencing results

High-throughput, plasmid-to-data solution for rapid and interactive construct validation

Same price across all plasmids, regardless of size and complexity

Low-cost, unbiased coverage for use in selection of complete, valid plasmid candidates

Chosen provider for all top 30 biopharma

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NGS Platforms

Azenta Life Sciences is a service provider of cutting-edge NGS platforms. For information on our NGS platforms and recommended configurations of your projects, please visit the NGS Platforms page. Azenta does not guarantee data output or quality for sequencing-only projects.

Illumina
PacBio
10x Genomics
NanoString
Nanopore
Olink


How To Order


Email | Phone +49 (0)341 520 122-41