SYNTHETIC DNA LIBRARIES

 

Researchers working in fields such as protein engineering, discovery biology, structural biology, synthetic biology, antibody engineering, and enzyme engineering choose Azenta Life Sciences Synthetic DNA Libraries because of our wide range of library types, reliable service, and best-in-class consultation from our Ph.D.-level customer support scientists. With competitive pricing and project completion in as little as 11 days, discover why hundreds of scientists from leading research facilities choose Azenta for their partially (NNK) or completely randomized (NNN) nucleotide libraries.

Applications

Protein Engineering: Build and identify proteins with novel or improved stability and functional characteristics from a library of mutants.

Enzyme Engineering: Modify an enzyme for enhanced activity for use in industrial biotechnology.

Directed Evolution: Build a library of variants with your desired mutations for applications  in evolutionary biology.

Synthetic Biology: Build new metabolic pathways with different combinations of genetic elements for applications in metabolic engineering.

Antibody Development: Test and identify mutant sequences with improved antigen expression.

CRISPR/Gene Editing: Create knock-out/knock-in variants for high-throughput screening.

DNA Library Services

Combinatorial (Classical)

Create a library of completely randomized (NNN) or partially randomized (NNK/NNS) mutants with multiple, different combinations of genetic elements.

Trimer-Controlled (Smart)

Specify an exact combination of codons, a precise percentage of desired amino acids (as low as 5%) and reduce frameshift mutations for the best mutant integrity.

Variant

Introduce singular/scanning site-directed mutagenesis in a user-defined 24 base pair region or truncate a sequence to characterize and optimize the desired protein features and function.

sgRNA

Accelerate CRISPR screening programs with single guide RNA (sgRNA) libraries containing knock-out/knock-in variants. Choose from pre-made or custom generated offerings.

Recombinant Antibody Production

Deliver life-saving therapeutics to market faster than ever before

Featured Application: Antibody Development

The success of an in-vitro antibody discovery and development program is contingent on the diversity of functional candidates as well as the ability to enhance favorable characteristics. Choose Azenta’s combinatorial or trimer-controlled libraries to expedite your antibody discovery and development program while keeping the cost low.

  • Combinatorial DNA libraries A powerful tool for determining and optimizing biomolecule function from a parent sequence. By coupling these libraries with a screening technology such as phage display, it becomes possible for researchers to both develop and test an immense number of mutant variants in a short time. Depending on the research goals, budget, and timeline, different mutagenesis techniques can be used to create variants. The below table compares three industry standard approaches. 

Library Stop Codons Risk of Mutation 'AT' Rich Bias  Assigned Amino Acids   Screening Speed Screening Costs

Random (NNN)

3 High Possible No Slow $$$

Partially Random (NNK/NNS)

1 High Possible No Moderate $$

Trimer-Controlled

0

Low

 None Yes Fastest $
  • Trimer-controlled libraries: An effective tool that significantly reduces screening efforts compared to conventional mutagenesis methods, empower researchers with the most accuracy, precision, and control as compared to other combinatorial DNA library techniques because of the extra synthetic effort devoted to restricting codon building blocks. Download the case study below to learn why researchers at a large bio-pharmaceutical company chose Azenta combinatorial trimer-controlled synthesis of antibody libraries to obtain a highly accurate mutant library.

Library Deliverables

Library Type Classical Combinatorial Libraries (NNN/NNK/NNS) and Trimer- Controlled (Smart libraries)
Variant

Library Format

Linear DNA pool Cloned Pool Individual Clones

Diversity

 Up to 1012 variants Up to 109 variants Customer defined
 Vector  N/A  Customer defined Customer defined
Quality Control Sanger sequencing of up to 96 individual transformants (default) 

Additional NGS-based diversity analysis (optional) 
Sanger sequencing of up to 96 individual transformants (default) 

Additional NGS-based diversity analysis (optional) 
100% sequence accuracy guaranteed.- Restriction digest of final plasmid 
Deliverables 1-2 µg of linear dsDNA 

Certificate of Analysis (COA) with quality measurements.
5-10 µg of plasmid DNA 

Certificate of Analysis (COA) with quality measurements. 

Large scale DNA preparation options ranging from µg to mg.
2-5 µg of plasmid DNA (standard) 

Certificate of Analysis (COA) with quality measurements. 

Large scale DNA preparation options ranging from µg to mg.

If you have custom requests or require any pre-project consultation, please contact our expert project management team at PM@azenta.com

Features & Benefits

Highly reliable: Researchers at leading pharma, biotech, and academic institutions rely on Azenta for their synthetic DNA library needs.
Improved accuracy: More precise control over library diversity allows you to overcome the inefficiencies of traditional approaches.
Convenience: Whether you need a high-diversity synthetic DNA library or a small rationally-designed variant library, Azenta can cater to all your DNA library needs, no matter the complexity or size.
High diversity: Achieve library diversity of 109 to 1012 depending on the type of project deliverable.
Quality control: Rigorous quality checks include statistical analysis and sequencing to ensure library diversity and quality.
Dedicated Ph.D.-level support at every step with real-time project updates through our online system.

HOW TO ORDER

*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service. Note that direct-sequencing templates are not available for our Same Day service.


Email | Phone: +49 (0)341 520 122-41