Synthetic DNA Libraries


Researchers working in fields such as protein engineering, discovery biology, structural biology, synthetic biology, antibody engineering, and enzyme engineering choose GENEWIZ’s Synthetic DNA Libraries because of our wide range of library types, reliable service, and best-in-class consultation from our Ph.D.-level customer support scientists. With competitive pricing and project completion in as little as 11 days, discover why hundreds of scientists from leading research facilities choose GENEWIZ for their partially (NNK) or completely randomized (NNN) nucleotide libraries.


Features & Benefits

Highly reliable: Researchers at leading pharma, biotech, and academic institutions rely on GENEWIZ for their synthetic DNA library needs
Improved accuracy: More precise control over library diversity allows you to overcome the inefficiencies of traditional approaches
Convenience: Whether you need a high-diversity synthetic DNA library or a small rationally-designed variant library, GENEWIZ can cater to all your DNA library needs, no matter the complexity or size
High diversity: Achieve library diversity of 109 to 1012 depending on the type of project deliverable
Quality control: Rigorous quality checks include statistical analysis and sequencing to ensure library diversity and quality

Featured Synthetic DNA Libraries

1. Alanine Scanning Library: Alanine scanning is a widely-used approach that involves substituting non-alanine residues sequentially with alanine within a customer-determined region by site-directed mutagenesis. Alanine is usually the preferred choice for a scanning library because substitution with alanine eliminates side-chain interactions while retaining the structural integrity of the protein because it is the smallest chiral amino acid. Cysteine scanning is another technique that researchers use for certain studies.

alanine scanning libraries

2. Combinatorial Library: A combinatorial library allows you to create a library of mutants by using different genetic elements in multiple combinations to determine the most beneficial combination. You can obtain a combinatorial library using traditional partially randomized (NNK/NNS) or completely randomized (NNN) approaches. To achieve even greater control over the amino acid distribution in your library, choose our trimer-controlled library solution to eliminate the introduction of unwanted codons and maximize the efficiency of downstream screening.

combinatorial library

3. Randomized Library: A randomized mutant library will allow you to create random amino acid substitutions in a small user-defined region such as an ORF. These proteins can then be expressed and assayed for function to determine beneficial substitutions in protein engineering studies.

randomized library

4. Site-directed Mutagenesis Library: In a site-directed mutagenesis library, a customer-determined amino acid position will be substituted sequentially with all the other 19 naturally-occurring amino acids to create a library of mutants. With this approach, you can use structural-functional analysis to examine the benefits or adverse effects of a particular amino acid substitution.

featured synthetic libraries

5. Truncation Library: A truncation library of mutants is created by trimming amino acids systematically from either the N- or C-terminus (or both), of a protein around a pre-defined core region. This allows you to identify the core region responsible for protein function.

truncation library

Available options for project deliverables:

Ready-to-clone PCR Fragment Library Cloned Pooled Library Individual Clones Library
  •  Up to 1012 variants
  •  Up to 109 variants
 Individual, 100% sequence-verified clones in your choice of vector

Quality Control:

  • Sequence verification of a pre-determined number of clones (with statistical analysis) based on library size
  • QC can be customized according to customer specifications+
+Additional charges may apply

Quality Control:

  • Sequence verification of a pre-determined number of clones (with statistical analysis) based on library size
  • QC can be customized according to customer specifications+
+Additional charges may apply

Quality Control:

  • Certificate of Analysis (COA) including restriction digest
  • Sequence trace data with alignment
  • Sequence files of the synthetic gene alone or subcloned into a vector


  • 1-2 µg of linear double-stranded DNA (ready-to-clone via 5’ and 3’ restriction sites if desired)


  • 5-10 µg of lyophilized plasmid DNA
  • Glycerol stock of total library with up to 10transformants


  • 2-5 µg of lyophilized plasmid DNA

Certain libraries may only be available as individual clones.

If you have custom requests or require any pre-project consultation, please contact our expert project management team at


Protein Engineering: Build and identify proteins with novel or improved stability and functional characteristics from a library of mutants

Enzyme Engineering: Modify an enzyme for enhanced activity for use in industrial biotechnology

Directed Evolution: Build a library of variants with your desired mutations for applications  in evolutionary biology

Synthetic Biology: Build new metabolic pathways with different combinations of genetic elements for applications in metabolic engineering

Antibody Development: Test and identify mutant sequences with improved antigen expression

Explore our recent citations to learn more about how top institutions are working with GENEWIZ to complete their research.


*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service. Note that direct-sequencing templates are not available for our Same Day service.

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