Digital spatial profiling (DSP) allows you to view your tissue in a new context by combining high-plex gene expression profiling with the spatial resolution of immunohistochemistry. This technology is a powerful tool for examining cellular interactions, tissue heterogeneity, pathogenicity, and response to therapy.
GENEWIZ Multiomics & Synthesis Solutions from Azenta Life Sciences is proud to be one of the first service providers to offer DSP utilizing the NanoString® GeoMx® Digital Spatial Profiler platform. This novel technology performs simultaneous, in situ spatial analysis of 570+ proteins and 18,000+ mRNAs from a single FFPE or frozen tissue section. Researchers can select from a host of discovery-focused panels with the potential for customization, including the new, comprehensive IO Proteome Atlas (IPA) and Whole Transcriptome Atlas (WTA) panels. Together with our epigenetic and single-cell solutions, GENEWIZ from Azenta equips researchers with the most cutting-edge technologies to study gene expression from every angle.
Digital spatial profiling (DSP) is a technique used to identify and quantify mRNA and protein expression in tissues such as FFPE (formalin-fixed paraffin-embedded) samples, fresh frozen tissue sections, tissue microarrays, and core needle biopsies.
Digital special profiling (DSP) is important for analyzing cellular heterogeneity and intracellular interactions, especially in the context of drug therapy response. Important advantages of DSP:
Azenta’s digital spatial profiling service on the GeoMx platform enables morphologically-guided gene expression profiling of FFPE or fresh frozen tissues using one of NanoString’s expertly-curated, predesigned panels. Researchers can provide tissue blocks or slides for processing, or Azenta can leverage its network of biobank partners to source high-quality, consented samples on your behalf.
Tissue is stained using a protein or mRNA panel and immunofluorescent markers
Regions of interest (ROIs) are selected using the GeoMx software (see FAQs)
Barcodes are cleaved using UV light and captured
Barcodes from each ROI are deposited in separate tubes
ROI-specific barcodes are quantified to create expression profiles
In this webinar presented by Douglas Hinerfeld, Ph.D., Director of Product Applications at NanoString, learn how their novel GeoMx platform simultaneously analyzes dozens of proteins and transcripts with high spatial resolution, providing greater insight into biological systems and diseases across a variety of research areas.
With this two-part webinar series, go beyond traditional transcriptomics and learn about the various NGS approaches available for gene expression analysis. In part 1, we take an in-depth look at various gene expression approaches, including RNA-Seq, single-cell RNA-Seq, digital spatial profiling, and more. In part 2, we explore the data generated from these approaches and how they can complement each other and confirm findings.
In this webinar first presented at Oxford Global's Spatial Biology US: Online conference, learn about the power of spatial genomics as presenter David Corney, Ph.D. presents the use of digital spatial profiling in performing high-plex protein profiling of gastric cancer and high-throughput RNA spatial profiling of urothelial carcinoma utilizing the NanoString® GeoMx™ Digital Spatial Profiler.
In this webinar, learn how spatial biology technology enables discovery of unique findings as Dr. Andres Matoso from Johns Hopkins University discusses his research in sarcomatoid urothelial carcinoma and his unique findings utilizing Azenta’s digital spatial profiling (DSP) services.