Epigenetic regulation includes modulating the transcriptional accessibility of genes through nucleosome positioning and chromatin remodeling. Assay for transposase-accessible chromatin sequencing (ATAC-Seq) identifies regions of open chromatin and efficiently labels them for high-throughput sequencing. ATAC-Seq has a higher signal-to-noise ratio and lower input requirements than other similar approaches. GENEWIZ now offers the entire ATAC-Seq workflow, from library preparation to data analysis.


ATAC-Seq Workflow

1. Tn5 Transposase Treatment

Cleave and tag open

2. Library Preparation

Purify fragmented DNA and amplify

3. Sequencing

Sequencing on Illumina® platform

4. Data Analysis

Alignment, peak calling, and differential analysis

Features & Benefits

Superior data quality: Guarantee of ≥80% bases with ≥Q30 (>90% on average)

Comprehensive bioinformatics for ATAC-Seq data

Dedicated Ph.D.-level support throughout the project

Industry-leading turnaround time with sequencing as fast as 1 week
More reasons to choose GENEWIZ

ATAC-Seq Overview

Assay for transposase-accessible chromatin sequencing (ATAC-Seq) employs a hyperactive form of Tn5 transposase to identify regions of open chromatin, which are important for global epigenetic control of gene expression. Tn5 simultaneously cleaves and adds adapters to nucleosome-free regions of DNA, priming them for sequencing.

How To Order

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