Epigenetic regulation includes modulating the transcriptional accessibility of genes through nucleosome positioning and chromatin remodeling. Assay for transposase-accessible chromatin sequencing (ATAC-Seq) identifies regions of open chromatin and efficiently labels them for high-throughput sequencing. ATAC-Seq has a higher signal-to-noise ratio and lower input requirements than other similar approaches. GENEWIZ now offers the entire ATAC-Seq workflow, from library preparation to data analysis.
1. Tn5 Transposase Treatment
Cleave and tag open
2. Library Preparation
Purify fragmented DNA and amplify
Sequencing on Illumina® platform
4. Data Analysis
Alignment, peak calling, and differential analysis
Features & Benefits
Superior data quality: Guarantee of ≥80% bases with ≥Q30 (>90% on average)
Comprehensive bioinformatics for ATAC-Seq data
Dedicated Ph.D.-level support throughout the project
Industry-leading turnaround time with sequencing as fast as 1 week
Assay for transposase-accessible chromatin sequencing (ATAC-Seq) employs a hyperactive form of Tn5 transposase to identify regions of open chromatin, which are important for global epigenetic control of gene expression. Tn5 simultaneously cleaves and adds adapters to nucleosome-free regions of DNA, priming them for sequencing.