ATAC-Seq

 

Epigenetic regulation includes modulating the transcriptional accessibility of genes through nucleosome positioning and chromatin remodeling. Assay for transposase-accessible chromatin sequencing (ATAC-Seq) identifies regions of open chromatin and efficiently labels them for high-throughput sequencing. ATAC-Seq has a higher signal-to-noise ratio and lower input requirements than other similar approaches. GENEWIZ now offers the entire ATAC-Seq workflow, from library preparation to data analysis.

Now accepting plant tissue!


  

ATAC-Seq Workflow

1. Sample Preparation

Isolate and freeze cells with protocol provided by GENEWIZ

Dead Cell Removal

2. Dead Cell Removal

If low viability, live cells are enriched using a magnetic bead-based approach

Library Preparation

3. Nucleic Extraction & Library Preparation

Optimized ATAC assay produces robust signal from low inputs

Sequencing

4. Sequencing

Guaranteed data quality exceeds Illumina® benchmarks

Bioinformatics

5. Bioinformatics

Alignment, peak calling, and multiomic data integration

Features & Benefits


Convenient sample preparation – freeze and store cells prior to submission; fresh cells or isolated nuclei are not required

Improved data quality – higher signal-to-noise ratio compared to traditional ATAC-Seq

Flexible starting material – submit as few as 50,000 cells; dead cell removal improves samples with low viability

Single-cell analysis available using the 10x Genomics® Chromium™ platform
More reasons to choose GENEWIZ

ATAC-Seq Overview

Assay for transposase-accessible chromatin sequencing (ATAC-Seq) employs a hyperactive form of Tn5 transposase to identify regions of open chromatin, which are important for global epigenetic control of gene expression. Tn5 simultaneously cleaves and adds adapters to nucleosome-free regions of DNA, priming them for sequencing.

How To Order


Email | Phone (1-877-GENEWIZ (436-3949), Ext. 1)