High-Throughput Gene Expression Screening
High-throughput gene expression (HT-GEx) screening is an RNA-Seq-derived approach we developed for optimal drug discovery and analysis, similar to DRUG-Seq. Unlike microarrays, our method offers rapid, unbiased, 3′ gene expression screening, providing complete cell-to-data analysis of the transcriptome at a lower cost than standard RNA sequencing. Working directly from lysate, GENEWIZ High-Throughput Gene Expression Screening works directly from cell lysate, saving both time and money by removing the need for RNA purifications. Additionally, the method performs 3’ transcript tagging to reduce required sequencing depths.
A Guide to RNA Sequencing
An in-depth guide for achieving success in your RNA-Seq experiments
High-Throughput Gene Expression Screening Applications
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Drug Discovery
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Compound Treatment Phenotyping
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CRISPR Treatment Phenotyping
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Cell Response Screening
HIGH-THROUGHPUT GENE EXPRESSION SCREENING WORKFLOW
High-Throughput Gene Expression Screening Assay Comparison
| GENEWIZ Service | Reads/Sample | Min. Sample Size | Cost | Starting Material | Selection Method | Detection Level | Gene Expression | Variant Detection | Alternative Splicing | Transcriptome Assembly |
|---|---|---|---|---|---|---|---|---|---|---|
HT-GEx Screening |
~1-2M Reads | 96/384 | $ | Cell Lysate | Poly(A) + | 3′ Tails of Genes | Suitable method | Not a suitable method | Not a suitable method | Not a suitable method |
mRNA-Seq |
~10-30M Reads | 1 | $$ | >20ng Total RNA | Poly(A) + | Poly(A) Transcript | Included | Included | Included | Included |
Total RNA-Seq |
~20-45M Reads | 1 | $$$ | >100ng Total RNA | rRNA – | All Transcript | Included | Included | Included | Included |
Iso-Seq |
~1-2M Reads | 1 | $$$$ | >1ug Total RNA | Poly(A) + | Full-Length Poly(A) Transcript | Not a suitable method | Not a suitable method | Preferred method | Preferred method |
High-Throughput Gene Expression Screening Deliverables
Standard Deliverables
- FASTQ files
- Quality summary report
Advanced Analysis
- UMI extraction, mapping, counting, and transformation
- Normalization
- Differential gene expression and clustering by compound/treatment
Custom bioinformatics analysis and reports are available. Please contact us about how we can customize the analysis to answer your biological question.
High-Throughput Gene Expression Screening Features & Benefits
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Fast Turnaround Times
Starting at just 2 weeks for quick target discovery
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High-Throughput Capacity
To rapidly scale up pilot or discovery projects
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Superior Data Quality
Exceeding manufacturers’ benchmarks
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High-throughput, cell-to-data solution for compound screening
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Low-cost, unbiased coverage for use in selection of drug candidates
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Receive detailed expression data for each well across multiple 96- or 384-well plates
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Free technical consultation and customer support from Ph.D.-level project managers and lab scientists
Technical Resources
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Webinar Series | Advancing Transcriptomics: Gene Expression Screening, Single-Cell RNA-Seq, and Beyond
With this two-part webinar series, go beyond traditional transcriptomics and learn about the various NGS approaches available for gene expression analysis. In part 1, we take an in-depth look at various gene expression approaches, including RNA-Seq, single-cell RNA-Seq, digital spatial profiling, and more. In part 2, we explore the data generated from these approaches and how they can complement each other and confirm findings.
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Tech Note | Achieving Phenotypic Profiling for Rapid Drug Discovery with High-Throughput Gene Expression Screening
High-throughput technologies are critical in performing phenotypic profiling for drug discovery applications. In this tech note, GENEWIZ from Azenta Life Sciences discusses the challenges associated with traditional approaches, such as microarrays and RNA sequencing, and offers an optimized assay to achieve high-quality phenotypic profiling at a reduced cost for rapid drug discovery.
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Information Sheet | High-Throughput Gene Expression (HT-GEx) Screening
Download our information sheet for a brief overview of GENEWIZ from Azenta’s high-throughput gene expression screening service.
NGS Platforms
For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. GENEWIZ does not guarantee data output or quality for sequencing-only projects.
