Next Generation Sequencing
View our Sample Submission Best Practices Checklist.
Interactive Sample Submission Guidelines
Direct Shipping:
Please use an overnight delivery service such as DHL for all NGS samples. A printout of the confirmed quote must be included with the material.
All direct shipments should be sent to:
European Customers (non-UK):
ATTN: NGS LAB
Katja Zunkel
GENEWIZ Germany GmbH
Bahnhofstrasse 86
04158 Leipzig
Germany
In case of any queries regarding NGS sample submission, please contact our Logistics Team at logistics.europe@azenta.com or call +49 (0)341 520 122-41.
If you need technical support, please contact us at ngs.europe@azenta.com or call +49 (0)341 520 122-41.
UK Customers:
ATTN: NGS LAB
William Hart
GENEWIZ UK Ltd
Hope End, Takeley
Essex, CM22 6TA
United Kingdom
In case of any queries regarding NGS sample submission, please contact our Customer Service Team at DNASeqUK@azenta.com or call 01279 873 837.
If you need technical support, please contact us at ngs.europe@azenta.com or call +49 (0)341 520 122-41.
Ambient Temperature and Dry-Ice Shipment possible. Please contact our Project Management team to discuss your shipping requirements further.
Phone: +49 (0)341 520 122-41
Email: ngs.europe@azenta.com
DNA can be concentrated free of charge via SpeedVacTM. Additional charges will apply for concentration via column or other methods.
Whole Genome Sequencing / Low-Pass WGS / Shotgun Sequencing
- Sample Type: Genomic DNA
- Sample Purity (OD260/280): 1.8-2.0
- Recommended Quantity: >1.5 µg, >20 ng/µl
- Minimum Amount: 500 ng
- Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
- Shipment Method: Dry ice
Single-Cell ATAC-Seq
- Sample Type: Cryopreserved single-cell suspension according to Azenta-supplied protocol, submitted in duplicate
- Recommended Quantity: >1M cells in 1 mL
- Minimum Amount: 100,000 cells in 500 µL (Note: 1E6 cells are required for dead cell removal)
- Buffer: Cryopreservation media (e.g. cell culture media supplemented with 20% FBS and 10% DMSO)
- Shipment Method: Dry ice
ChIP-Seq
- Sample Type: ChIP DNA
- Sample Purity (OD 260/280): 1.8-2.0
- Recommended Quantity: >50 ng
- Minimum Amount: 10 ng
- DNA Fragment Length: 100-800 bp; 200-300 bp (Main peak)
- Resuspension Buffer: Water, BE, or low TE (<0.1 mM EDTA)
- Shipment Method: Dry ice
Agilent SureSelect Exome Enrichment Kit, OncoGxOne™ Discovery Cancer Panels and TargetGxOne™ Gene Enrichment
- Sample Type: Genomic DNA
- Sample Purity (OD260/280): 1.8-2.0
- Recommended Quantity: >1.5 µg, >20 ng/µl
- Minimum Amount: 500 ng
- Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
- Shipment Method: Dry ice
Custom TargetGxOne™ Amplicon Sequencing
- Sample Type: Genomic DNA
- Sample Purity (OD260/280): 1.8-2.0
- Recommended Quantity: >1 µg, >20 ng/µl
- Minimum Amount: 100 ng
- Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
- Shipment Method: Dry ice
16S MetaVx™ Panels/ITS 2
- Sample Type: Genomic DNA
- Sample Purity (OD260/280): 1.8-2.0
- Recommended Quantity: >1 µg, >20 ng/µl
- Minimum Amount: 100 ng
- Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
- Shipment Method: Dry ice
Pre-Made Sequencing Libraries
- Recommended Quantity: >15 nM
- Volume: ≥20 ul
- Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
- Shipment Method: Dry ice
genoTYPER-NEXT
- Sample Type (Preferred): Fresh/frozen cell pellet
- Recommended Quantity: ≥106 cells
- Required Quantity: ≥3x104 cells
- Shipment Method: Dry ice
- Sample Type: Genomic DNA
- Sample Purity (OD260/280): 1.8-2.0, RNA-Free
- Recommended Quantity: >1.5µg, >20 ng/µl
- Minimum Amount: 500 ng
- Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
- Shipment Method: Dry ice
Amplicon-EZ
- Sample Type: Purified PCR Products
- Sample Purity (OD260/280): 1.8-2.0
- Concentration: 20 ng/µl
- Minimum Amount: 500 ng
- Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
- Shipment Method: Room temperature/blue ice/dry ice
RNA Sample Submission Guidelines
We now offer RNA Stabilization Tubes. Ship RNA safely at ambient temperature. Download the information sheet. Contact us to receive a free trial.
Standard/Strand-Specific RNA-Seq & Immuno-Profiling
- Sample Type: Total RNA
- Sample Purity (OD260/OD280): 1.8-2.2
- Recommended RIN: ≥6.0
- Recommended Quantity: >2 µg, >50 ng/µl
- Minimum Quantity: 500 ng
- Resuspension Buffer: Nuclease-free water
- Shipment Method: Dry ice
Small RNA-Seq
- Sample Type: Total RNA (containing small RNA) in nuclease-free water, on dry ice
- Sample Purity (OD260/OD280): 1.8-2.2, DNA-free, ≥ 50 ng/μL, 50 ng/μL
- Recommended RIN: ≥6.0
- Recommended Quantity: Total RNA: ≥2 µg, >50 ng/µl, purified small RNA: >50 ng, >5 ng/µl
- Resuspension Buffer: Nuclease-free water
- Shipment Method: Dry ice
Ultra-Low Input RNA-Seq
Cell number and buffer clearly indicated in Azenta Sample Submission Form
- Sample Type: Cells in solution or small amounts of total RNA
- Recommended Quantity: >1,000 cells/sample in Trizol or 20 ng of total RNA
- Shipment Method: Dry ice
Single-Cell RNA-Seq & Single-Cell Immuno-Profiling
- Sample Type: Frozen cell suspension according to Azenta-supplied protocol, submitted in duplicate. Methanol-fixed cells are also accepted.
- Sample Viability: >90% post-thaw
- Recommended Quantity: >1M cells in 1 mL
- Minimum Quantity: 50,000 cells in 500 µL
- Resuspension Buffer: Appropriate cryopreservation media (e.g., cell culture media supplemented with 20% FBS and 10% DMSO)
- Shipment Method: Dry Ice overnight
High-Throughput Gene Expression (HT-GEx) Screening
- Recommended Quantity: Frozen cell lysates, 5000 - 20,000 cells in 15µl of lysis buffer
- Recommended Lysis Buffer: QIASeq UPX Cell Lysis Buffer (#333076) or equivalent
- Shipment Method: Dry ice
- Sample Container: PCR-clean 96-well PCR-plate -80°C dry-ice resistant and foil sealed
- Sample Type:
- Cell lysate
- Purified RNA (please contact us for details)
PacBio Iso-Seq
- Sample Type: Total RNA
- Sample Purity (OD260/280): 1.8 - 2.2
- Recommended RIN: ≥9.0
- Recommended Quantity: ≥2µg
- Recommended Volume: 10 - 20µL
- Shipment Method: Dry ice
Protein Sample Submission Guidelines
Olink Proteomics®
Sample Preparation:
- Recommended Quantity: 30µl - 100µl at 0.5 µg/µL – 1.0 µg/µL of total protein concentration
- Minimum Amount: 20µl
- Shipping Method:Dry ice
- Sample Container:PCR-clean tubes or 96-well PCR plate -80°C dry-ice resistant and easily resealable
- Sample Type:EDTA-plasma, interstitial fluids, cell lysate, microdialysis fluids, microvesicles, citrate-plasma, dried blood spots, synovial fluids, final needle biopsies, urine, saliva, tissue lysates, serum, cebrospinal fluids, cell culture media, heparin-plasma, plaque extracts
All extractions are performed to best of Azenta’s ability and the Client is charged for work performed regardless of outcome. Additional charges for procurement of kits may apply for select extractions. Please Note: We do not accept samples above BSL 2 and we require prior notification for pathogenic samples.
FFPE Samples
- Recommended Quantity: ≥4 FFPE slides recommended
- Minimum Quantity: 2 FFPE slides
- Thickness: 5µm-20 µm
- Area: >150 mm2
- Shipping Method: Room temperature/blue ice
Cell pellets
- Recommended Quantity: 106 cells
- Minimum Quantity: 104 cells
- Shipping Method: Dry ice
Fresh Frozen Tissue
- Recommended Quantity: 10 mg
- Minimum Quantity: 2 mg
- Shipping Method: Dry ice
Viral Particles
- Recommended Quantity: >1 mL supernatant
- Viral Titer: >5x109 cells/mL
- Shipping Method: Dry ice
Whole Blood
- Recommended Quantity: 2 tubes/sample
- Recommended Volume (DNA Extraction): 500 µl/tube
- Minimum Volume (DNA Extraction): 200 µl/tube
- Recommended Volume (RNA Extraction): 2.5 mL (whole blood, PAXgene Blood RNA Tube)
- Shipping Method: Fresh: Blue ice, frozen: Dry ice
Plasma/Serum
- Recommended Quantity: 2 tubes/sample
- Recommended Volume: 10 mL/tube
- Minimum Volume: 5 mL/tube
- Shipping Method: Dry ice
Stool
- Recommended Quantity: 100 mg
- Minimum Quantity: 50 mg
- Shipping Method: Dry ice/blue ice
Saliva
- Recommended Quantity: 1 mL
- Shipping Method: Dry ice/blue ice
Soil
- Recommended Quantity: 100 mg
- Minimum Quantity: 50 mg
- Shipping Method: Room temperature/blue ice
Water
- Recommended Volume: 100 mL
- Minimum Volume: 20 mL
- Shipping Method: Room temperature
Swab
- Recommended Quantity: 2 tubes/sample. 1 swap/tube
- Shipping Method: Room temperature
Fixed Needle Core Biopsy
- Recommended Quantity: 4 biopsies
- Minimum Quantity: 1 biopsy
- Recommended Diameter: 1-2 mm diameter; >5 mm in length
- Shipping Method: Room temperature/blue ice
Snap Frozen Needle Core Biopsy
- Recommended Quantity: 10 mg
- Minimum Quantity: 3 mg
- Shipping Method: Dry ice
Azenta Sample Quality Control Assessment
Azenta performs QC free of charge with results reported within 24 – 48 hours.
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DNA* |
RNA |
Pre-Made Libraries |
TapeStation |
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Qubit Assay |
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*For High Molecular Weight DNA, gel electrophoresis will also be performed.
For questions on submitting other, or less than ideal sample types, please contact our Project Management team to discuss further as we may be able to proceed with your project.
Phone: +49 (0)341 520 122-41
Email: NGS.europe@azenta.com