Azenta offers two service types for Sanger sequencing of PCR products or plasmids: Pre-Mixed or Pre-Defined, both available for tubes and plates. Our barcode-driven automated workflows enable flawless and convenient sample processing. To determine the service that is best for your samples, please see the table and the descriptions for each service below. To ensure the best results, please submit your samples according to our Sample Submission Guidelines for each service type.
If you need help choosing your optimal Sanger service option, please contact us:
For sequencing plasmids or purified PCR products, this service is the most economical choice. You provide your template and primer mixed in the same tube.
You submit your template in a tube and we will mix template and a defined primer when your samples are received. You can choose from different available FREE Universal Primers or provide your own, labeled with a Purple, primer barcodes
This is a full-service option for sequencing of purified plasmids and purified PCR product samples with unknown concentration. For purified plasmids column-based PCR purifications, a GENEWIZ scientist will measure and adjust the concentration of your template to optimize the sequencing reaction.
Note that if you have enzymatically-purified PCR products, we recommend that you submit these samples under the Pre-Mixed or Pre-Defined service levels. An accurate OD cannot be obtained from an enzymatically-purified template.
Submit template and primer separately according to our Sample Submission Guidelines.
Note: A GENEWIZ Universal Primer can be used in this service at no charge.
Fully-automated workflow processes, quick turnaround times
Easy online ordering with no need for submitting complicated order forms
Reliable way to accurately read encoded information, eliminating the possibility of human error
Safe and secure data download from your CLIMS account