LENTIVIRUS PRODUCTION SERVICES

Lentiviral vectors are broadly used in laboratories across academia and industry for research and clinical gene therapy applications. Gene therapy vectors derived from lentivirus offer an array of unique advantages over traditional retroviral gene delivery systems. Leading these is their ability to provide long-term and stable gene expression and robust infection of both dividing and non-dividing cells, such as neurons.

With a comprehensive set of lentiviral solutions that span from construct synthesis through viral production and even include storage of transduction-ready virus, you can enjoy the convenience of an end-to-end solution to advance your research. Contact an expert to discuss your specific lentivirus needs.

Lentivirus Production For Cell and Gene Therapy

Starting at €488 / £473

Lentiviral vectors are research tools used to introduce gene products into in vitro systems, animal models, or human patients via gene therapy. Lentiviruses have a broad range of applications and are commonly used in clinical gene therapy as well as in CAR-T cell engineering.

Advantages of Lentivirus

Lentiviral vectors can carry relatively large transgenes
Low immunogenicity
Can be used for gene editing (e.g. deliver CRISPR elements to the cell)
Ability to transduce both dividing and non-dividing cells
Integration into the host genome and stable expression of the transgene

Lentivirus Production Workflow

Azenta Advantages

Convenient one-stop shop from gene synthesis or plasmid prep to lentivirus packaging
Variety of viral prep scales with high-titer transduction-ready virus available

Functional titering option available.
Ph.D. scientists to support you with project management

Large selection of lentiviral vectors available

Component	2nd Generation	3rd Generation
Transfer Plasmid	Can be packaged ONLY by a second-generation packaging system	Can be packaged by both 2nd and 3rd generation packaging systems
Packaging Plasmid	Plasmids includes gag, Pol, Rev, TAT for entry and integration of the viral genome	Plasmids include only packaging genes gag and Pol
Envelope Plasmid	Contains gene encoding for envelope proteins	Contains only envelope gene Env
Safety	Safe. Replication incompetent: uses 3 separate plasmids encoding various HIV genes	Safer. Transfer plasmids are all replication incompetent rendering the virus “self-inactivating” (SIN) after integration.
Titer	Higher viral yield	High viral yield

Lentivirus Production Offerings

Virus Titer	Volume Available	QC
>10⁷ IFU/mL	250µL - 500µL, 1mL - 1.5mL	Physical and functional titering options are available
>10⁸ IFU/mL	250µL - 500µL, 1mL - 1.5mL
>10⁹ IFU/mL	250µL - 500µL, 1mL - 1.5mL