CLIA Sanger Sequencing


GENEWIZ provides CLIA Sanger sequencing as the clinical "gold standard" from our state-of-the-art CLIA-certified and CAP-accredited laboratory. This standalone sequencing service utilizes a CLIA-compliant workflow to support your clinical diagnostic assays, leading to advancements in personalized medicine and molecular diagnostics.

CLIA Sanger sequencing is best utilized as the initial readout from a PCR assay or in conjunction with next generation sequencing, specifically to elucidate complex regions not approachable by other methodologies or to confirm variants uncovered by genome-wide techniques.

Analyze thousands of genetic markers at once

Microarray Solutions



Features And Benefits

High Quality Data: Typical read will provide 800 bases Phred20
Long Sequencing Reads: DNA sequencing read lengths up to ~1000 bases

Proprietary Protocols: Best-in-class protocols to sequence difficult RNAi and GC-rich templates
Fast Turnaround Time: Results available within two to three business days

CLIA Sanger Sequencing Service Options


For sequencing plasmids or purified PCR products, provide your template and primer mixed in the same tube for the most economical CLIA-compliant services.


If choosing our Pre-Defined Service, submit your plasmids or purified PCR products and primer in separate tubes, and a GENEWIZ scientist will mix them once your sample arrives on site.


Full-service option allows you to submit unpurified PCR product with unknown concentration along with your primer for sequencing. We will purify your PCR product as well as measure and adjust the concentration to optimize your reactions.

View our Sample Submissions Guidelines

Template Types

Purified Plasmids or PCR Products

Purified Plasmids or PCR Products

Un-Purified PCR Products

Un-Purified PCR Products

Difficult Template Sequencing

Difficult Template Sequencing


All customers will receive their raw data as .ab1, .seq, and .phd files along with a quality score report.

Troubleshooting DNA Templates
with Sanger Sequencing




*Samples must arrive at the GENEWIZ New Jersey Laboratory before 10:00 am EST to qualify for Same Day service. Note that direct-sequencing templates are not available for our Same Day service.

Email | Phone (1-877-GENEWIZ (436-3949), Ext. 3350)