PLASMID DNA PREPARATION

 

Delivering high quality DNA from a variety of starting materials in a cost-effective and reliable manner is our priority. Don't waste your valuable time with a plasmid preparation kit when GENEWIZ can conveniently provide plasmid DNA preps at the scale and quantity you need.

Our standard plasmid DNA preparation service is a flexible and fast choice for a range of plasmid templates. Runs are available in the classic mini to giga scales, yielding micrograms to milligrams of purified plasmid DNA. Additional add-on service and analysis options are available, enabling project customization to meet budgetary and technical specifications.


Features & Benefits

No plasmid purification kit needed – Submit starting material and GENEWIZ will deliver your purified plasmid product
Fast delivery time - Rapid turnaround time, starting at just two business days, to meet your research deadlines
Convenient online ordering - Place an order directly through your GENEWIZ account, including your Sanger sequencing results page
Quality control – Strict QC process with multiple analysis options to ensure high-quality plasmid prep

 

Plasmid DNA Prep Service & Analysis Options

= Included

= Add-on

Turnaround Time Accepted Plasmids Service Options Analysis Options

Starting from 2-3 business days

  • High- or Low-Copy
  • Toxic Construct
  • Stable or Unstable
Spectrophotometric DNA Quality Analysis
Minimum Yield Verification
Transfection-Grade (Endotoxin Free)
DNA Normalization
Aliquots or Custom Tubes
GLP-Compliant
DNA Sequencing
Sequence Consensus Assembly & Analysis
Full-Service DNA Sequence Confirmation
Restriction Digest & Agarose Gel Analysis
Sterility Testing

Not all options available for mini-scale standard and 96-well preps. Please contact MB@genewiz.com to discuss your miniprep project.

Upgrade to our amount based Targeted Yield Service. Transfection-grade and value-added analysis options included.


DNA PREPARATION SCALES AND YIELDS

Run Scale Typical Yield for High-Copy, Stable Plasmids^ Typical Yield for Low-Copy, Unstable, or Toxic Plasmids^
Midi 70-100 μg 10-50 μg 
Maxi 350-500 μg 50-250 μg 
Mega 1.5-2.5 mg 250 μg-1.25 mg 
Giga 7-10 mg 1-5 mg
Mini Preps Typical Yield for High-Copy, Stable Plasmids^ Typical Yield for Low-Copy, Unstable, or Toxic Plasmids^
Tube 7-10 μg 2-5 μg
96-well Plates 5-10 μg 1-3 μg

^Actual yield will vary with the nature of the plasmid, bacterial hosts, and other factors. Please contact MB@GENEWIZ.com to discuss known low-copy, unstable, or toxic constructs.

Choice of Starting Material

Building

DNA

Transformation provided as a service*

Building

Glycerol Stock

Provide 200 μL frozen stock

Building

Bacterial Colony

Please provide fresh colonies (<3 days old)

*For DNA samples, GENEWIZ provides multiple host strains to choose from for transformation. For custom strains, please provide the starting material as bacterial colony or clonal glycerol stock, or select “Other” for host strain when placing your order and include the competent cells with your DNA submission.

HOW TO ORDER

*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service. Note that
direct-sequencing templates are not available for our Same Day service.


Email | Phone (1-877-GENEWIZ (436-3949), Ext. 3) | Live Chat