ATAC-Seq is a technique used to analyze the functional state of chromatin at the genome-wide level. The assay for transposase-accessible chromatin sequencing identifies regions of open chromatin and efficiently labels them for high-throughput sequencing. Epigenetic regulation includes modulating the transcriptional accessibility of genes through nucleosome positioning and chromatin remodeling. ATAC-Seq has a higher signal-to-noise ratio and lower input requirements than other similar approaches. GENEWIZ Multiomics and Synthesis Solutions from Azenta Life Sciences offers the entire ATAC-Seq workflow, from library preparation to data analysis, and accepts plant tissue as starting material.
Single-cell ATAC-Seq (scATAC-Seq) identifies genome-wide chromatin accessibility at the individual cell-level to uncover how chromatin structure and DNA-binding proteins regulate gene expression in varying states and cellular processes.
ATAC-Seq Workflow
1. Sample Preparation
Isolate and freeze cells with protocol provided by Azenta
2. Dead Cell Removal
If low viability, live cells are enriched using a magnetic bead-based approach
3. Nucleic Extraction & Library Preparation
Optimized ATAC assay produces robust signal from low inputs
4. Sequencing
Guaranteed data quality exceeds Illumina® benchmarks
5. Bioinformatics
Alignment, peak calling, and multiomic data integration
ATAC-Seq Features & Benefits
Convenient sample preparation – freeze and store cells prior to submission; fresh cells or isolated nuclei are not required
Improved data quality – higher signal-to-noise ratio compared to traditional ATAC-Seq
Flexible starting material – submit as few as 50,000 cells; dead cell removal improves samples with low viability
Single-cell analysis available using the 10x Genomics® Chromium™ platform
Assay for transposase-accessible chromatin sequencing (ATAC-Seq) employs a hyperactive form of Tn5 transposase to identify regions of open chromatin, which are important for global epigenetic control of gene expression. Tn5 simultaneously cleaves and adds adapters to nucleosome-free regions of DNA, priming them for sequencing.