Gene Fragments are double-stranded, linear DNA fragments of 100-3,000 bp, which are a convenient option due to their faster delivery time, lower cost, and greater flexibility. These fragments are synthesized with the same industry-leading proprietary technology that Azenta Life Sciences, formerly GENEWIZ has developed for standard gene synthesis products. We provide Gene Fragments in a lyophilized form that can be easily re-suspended, cloned, and screened to identify the correct clone for downstream applications.
Construction begins with the base-by-base synthesis of oligonucleotides (oligos), followed by assembly into double-stranded DNA (dsDNA) fragments. These custom DNA fragments can be used directly, cloned into vectors, or assembled into larger constructs to serve a variety of research uses.
|Sequence Length*||Quantity||Completion Time**||Predicted Cloning Accuracy|
|100-500 bp||500 ng||2-4 business days||>85%|
|501-750 bp||3-5 business days|
|751-1,000 bp||1,000 ng|
|1,001-1,500 bp||4-6 business days|
|1,501-2,250 bp||6-8 business days|
*Only sequences with overall GC content between 20% and 80% qualify for Gene Fragments service. GC/AT rich or sequences with complex repeats do not qualify.
**Orders containing more than 50 fragments will require additional turnaround time.
Standard deliverables include 500-1,000 ng of lyophilized double-stranded DNA, depending on sequence length.