Plasmid Prep FAQs

Service Options

A:
GENEWIZ Plasmid Prep includes Standard service with mini to giga scale preps, and Targeted Yield service to deliver specific quantities of transfection-grade DNA. We also offer 96-well mini-preps and custom large-scale preps for your specific project needs. Plasmid prep can also be ordered using bacterial colonies or glycerol stocks from a Sanger sequencing order.
A:
GENEWIZ offers a wide variety of analysis options with your plasmid prep order including DNA sequencing, DNA normalization, restriction digest analysis, sterility testing and much more.
A:
GENEWIZ has proven a steadfast commitment to superior service over the last two decades, becoming a global leader in genomics services. With plasmid DNA preparation, GENEWIZ offers rapid turnaround times starting at just 2 business days, convenient online ordering, and a strict QC process with a wide range of analysis options to ensure high quality.

Sample Submission

A:

  • Purified DNA: at least 5 µL of DNA at 200 ng/µL (minimum 100 ng). Additional charge applies.
  • Agar plate: fresh (<3 days old) plate with single, well isolated colonies. Customer may indicate the colony (or colonies) to use, if desired.
  • Glycerol stock or media sample: at least 200 µL. GENEWIZ can streak colonies (additional charges apply) for isolation prior to starting the liquid culture upon request.
  • Cell pellet: Ship frozen on dry ice (direct to GENEWIZ via overnight carrier), with an indication of the culture volume, media used, OD600 at harvest, and cell pellet mass.
  • DNA on filter paper: this format is discouraged – please send as purified, wet DNA in a sealed tube via overnight shipping (or GENEWIZ courier, if available). Additional charge applies.
  • Other samples: please contact GENEWIZ Technical Support to discuss custom requirements.
A:
We routinely stock Carbenicillin (Carb), Kanamycin, Chloramphenicol, Ampicillin, Tetracycline, Streptomycin, Spectinomycin, and Zeocin. We also supply Gentamycin, Hygromycin, Apramycin, and Erythromycins but do not keep them on hand, so some lead time will be necessary. An automatic upgrade of Ampicillin to Carbenicillin is included at no added charge. Other antibiotics may be submitted by the customer with their samples.
A:

Fresher is better; < 3days old with well isolated colonies. Older plates can be freshly streaked for isolation upon request. Please note that this will add to the order timeline.

Please use caution in shipping agar plates during the cold winter months. The agar media may freeze in transit, rendering the plate unusable. Glycerol stocks are recommended as a substitute format during this season.

A:
Our plasmid prep team accepts only BSL1 samples. Typical agar plates used for these strains, bearing well isolated colonies are usually acceptable.
A:
Our Plasmid DNA Preparation service is for plasmids in the 3-15 kb range. We do not currently offer BAC or cosmid preparation services.
A:
We do not currently offer BAC or cosmid preparation services. Plasmids requiring handling outside standard protocols are reviewed on a case-by-case basis. Additional charges may apply.
A:
Please send at least 10 µL for each sequencing reaction using that primer. The primer concentration should be 5 µM.
A:

Larger plasmids (>10 kb) and plasmids containing toxic or unstable sequences (such as long terminal repeats (LTR) found in viral expression vectors) are likely to produce better results when paired with cell lines engineered to maintain these difficult sequences.

We advise against using standard cloning strains for these plasmids, due to increased risk of lower yield and plasmid instability. We also suggest the selection of additional QC testing to verify unstable or toxic plasmids.

A:
Please contact GENEWIZ Technical Support at DNASeq@GENEWIZ.com with any questions and to discuss your project.

Turnaround Time and Troubleshooting

A:
Typical turnaround time is 2-4 business days, depending on the order options selected. Additional testing or repeats may extend the timeline.
A:
E-mail DNASeq@GENEWIZ.com, call 908-222-0711 ext.2, or live chat with our technical support team.
A:
Upon request, GENEWIZ will store a sample of your starting material or final DNA for up to two years at no additional charge, to facilitate subsequent ordering. Please be sure to request this storage in your order comments and include the order tracking number when reordering preps.
A:
GENEWIZ Project Management stores a sample of your final DNA from a gene synthesis order for up to one year at no additional charge. To order preps from these projects, simply go to the gene synthesis results page, select the construct of interest in the checkbox, and click “submit prep order” to initiate an online plasmid prep order.
A:

Our internal quality monitoring will trigger the automatic repeat of a plasmid DNA preparation when the yield is below a critical threshold, depending on the anticipated copy number for the plasmid. Yield variance above this threshold could be due to a variety of factors and may be intrinsic to the sample provided.

Plasmid DNA preparation orders are processed by run. For low copy or other low yield plasmids, we recommend ordering a larger preparation scale or multiple preps, depending on the amount of final material required.

To discuss troubleshooting or custom process modifications, please contact GENEWIZ Technical Support.

GLP-level Plasmid Preparation

A:
Yes. GENEWIZ’s Quality System is developed in accordance with applicable FDA GLP regulations described in Title 21, Part 58 of the Code of Federal Regulations and EPA GLP regulations described in Title 40, Part 160 of the Code of Federal Regulations. We offer GLP services including GLP Plasmid Prep. For more details, please contact us at 877-GENEWIZ (436-3949) ext. 3350 or Regulatory@genewiz.com.

Have a specific question?

Email | Phone (1-877-GENEWIZ (436-3949), Ext. 2) | Live Chat