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TransScript®-Uni One-Step gDNA Removal and cDNA Synthesis SuperMix

TransScript®-Uni RT is an improved version of M-MLV reverse transcriptase with broad range of reaction temperature(42°C-65°C) and higher thermostability. The suggested reaction temperature is 50°C. The SuperMix contains reagents for simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85°C for 5 seconds.

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Highlights

• Broad range reaction temperature (42°C - 65°C ) .                                                                                                                                 • Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.                                            • The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.                                                                                                                                                                                                                          • cDNA up to 20 kb.

Applications

• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template
• cDNA library construction, primer extension, 3’ and 5’ RACE

Storage

-20°C for two years

 

Kit Contents

Component

AU311-02

AU311-03

TransScript® -Uni RT/RI Enzyme Mix

50 μl

100 μl

gDNA Remover

50 μl

100 μl

2×TS-Uni Reaction Mix

500 μl

1 ml

Random Primer(N9) (0.1 μg/μl)

50 μl

100 μl

Anchored Oligo(dT)20 Primer (0.5 μg/μl)

50 μl

100 μl

RNase-free Water

500 μl

1 ml