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TransScript Probe One-Step qRT-PCR SuperMix TransScript Probe One-Step qRT-PCR SuperMix

TransScript Probe One-Step qRT-PCR SuperMix

TransScript® Probe One-Step qRT-PCR SuperMix is designed for one-step qRT-PCR with high sensitivity, high synthesis efficiency and high amplification efficiency. This kit firstly synthesizes first-strand cDNA with RNA as templates using reverse gene-specific primers, and then performs qPCR with the synthesized cDNA as templates using both forward and reverse gene-specific primers and fluorescent probes to achieve one step from reverse transcription to qPCR in a single tube.

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Product Details

TransScript® Probe One-Step qRT-PCR SuperMix is designed for one-step qRT-PCR with high sensitivity, high synthesis efficiency and
high amplification efficiency. This kit firstly synthesizes first-strand cDNA with RNA as templates using reverse gene-specific primers,
and then performs qPCR with the synthesized cDNA as templates using both forward and reverse gene-specific primers and fluorescent
probes to achieve one step from reverse transcription to qPCR in a single tube.

 

Highlights
• Simple to use with reduced contamination
• High sensitivity and high specificity

 

Applications
• High-copy and low-copy gene detection
• Detection of RNA virus or trace amounts of RNA

 

Storage
at -20°C for one year
 

Shipping
Dry ice (-70 ℃)

 

Product Contents

 

Component AQ221-01 AQ221-02
TransScript® Probe One-Step RT/RI Enzyme Mix 40 μl 160 μl
TransStart® Probe qPCR SuperMix (2×) 1 ml 4×1 ml
Passive Reference Dye (50×) 40 μl 160 μl
RNase-free Water 1 ml 4×1 ml

 

 

References

Li W, Mao L, Zhou B, et al. The swine CD81 enhances E2-based DNA vaccination against classical swine fever[J]. Vaccine, 2015, 33(30):3542-3548.