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EasyPure Viral DNA/ RNA  Kit EasyPure Viral DNA/ RNA  Kit

EasyPure Viral DNA/ RNA Kit

EasyPure® Viral DNA/RNA Kit utilizes a unique lysis buffer to lyse virus and release DNA / RNA. The released DNA / RNA is effectively purified after specifically binding to a silica-based spin column. It is suitable for isolating viral DNA/RNA from up to 200 μl of plasma, serum, whole blood, tissue homogenate, cell-free body fluid, nasopharyngeal or oropharyngeal aspirate/wash, bronchoalveolar lavage fluid (BALF), tracheal aspirate, sputum, nasopharyngeal or oropharyngeal swab and animal cell culture supernatant. The isolated DNA/RNA with high purity can be applied in PCR, RT-PCR, qPCR, qRT-PCR, etc.

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Product Details

EasyPure® Viral DNA/RNA Kit utilizes a unique lysis buffer to lyse virus and release DNA / RNA. The released DNA / RNA is effectively purified after specifically binding to a silica-based spin column. It is suitable for isolating viral DNA/RNA from up to 200 μl of plasma, serum, whole blood, tissue homogenate, cell-free body fluid, nasopharyngeal or oropharyngeal aspirate/wash, bronchoalveolar lavage fluid (BALF), tracheal aspirate, sputum, nasopharyngeal or oropharyngeal swab and animal cell culture supernatant. The isolated DNA/RNA with high purity can be applied in PCR, RT-PCR, qPCR, qRT-PCR, etc.

 

Storage
Proteinase K solution at -20°C for two years; others at room temperature (15°C-25°C ) for one year.
 

Shipping
Proteinase K on dry ice (-70 °C); others at room temperature.

 

Product Contents

 

Component ER201-01 (50 rxns) ER201-02 (200 rxns)
Binding Buffer 5 (BB5) 15 ml 60 ml
Wash Buffer 5 (WB5) 12 ml 2×24 ml
Proteinase K (20 mg/ml) 1 ml 4×1 ml
RNase-free Water 10 ml 20 ml
RNase-free Tube (1.5 ml) 50 each 200 each
RNA Spin Columns with Collection Tubes 50 each 200 each

 

 

References

Bingqing L , Yingying Y , Yajie Z , et al. A Novel Enterovirus 71 (EV71) Virulence Determinant: The 69th Residue of 3C Protease Modulates Pathogenicity[J]. Frontiers in Cellular and Infection Microbiology, 2017, 7:26.
Wu Y , Cui X , Wu N , et al. A unique case of human Zika virus infection in association with severe liver injury and coagulation disorders[J]. Scientific Reports, 2017, 7(1):11393.
Dongfang H , Lin L , Jinyuan G , et al. Genetic Diversity and Positive Selection Analysis of Classical Swine Fever Virus Envelope Protein Gene E2 in East China under C-Strain Vaccination[J]. Frontiers in Microbiology, 2016, 7:85.
Gao Y , Li Y , Meng Q , et al. Serum HBV DNA, RNA and HBsAg: which correlated better to intrahepatic covalently closed circular DNA before and after nucleos(t)ide analogue treatment[J]. Journal of Clinical Microbiology, 2017:JCM.00760-17.
Chen S , Luo G , Yang Z , et al. Avian Tembusu virus infection effectively triggers host innate immune response through MDA5 and TLR3-dependent signaling pathways[J]. Veterinary Research, 2017, 47(1):74.