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FlyCut® EcoRV

FlyCut® EcoRV is expressed and purified from E.coli that carries the recombinant EcoRV gene. The molecular weight is 29.5 kDa, with the recognition site at GAT^ATC. The reaction is conducted for 5-15 minutes at 37°C, and heat-inactivated at 65°C for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.

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Concentration

20,000 units/ml

Recognition Site

5’...GAT↓ATC...3’

3’...CTA↑TAG...5’

Applications

Genomic DNA, plasmid DNA, PCR product

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Storage Buffer

20 mM Tris-HCl pH 7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/ml BSA, 50% Glycerol

10× FlyCut® Buffer

500 mM Tris-Ac pH 7.9, 1 M KAc, 120 mM MgAc2, 1 mg/ml BSA

Storage

-20˚C for two years

Shipping Condition

Dry ice (-70˚C)

 

 

Kit Contents

Component JE301-01 JE301-02
FlyCut® EcoRI  2,000 units  2 × 2,000 units 
10× FlyCut® Buffer 500 μl  1 ml
10× DNA Loading Buffer 1 ml 1 ml