|
Concentration |
20,000 units/ml |
|
Recognition Site |
5’...T↓CTAGA...3’ 3’...AGATC↑T...5’ |
|
Applications |
Genomic DNA, plasmid DNA, PCR product |
|
Unit Definition |
One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
|
Storage Buffer |
20 mM Tris-HCl pH 7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/ml BSA, 50% Glycerol |
|
10× FlyCut® Buffer |
500 mM Tris-Ac pH 7.9, 1 M KAc, 120 mM MgAc2, 1 mg/ml BSA |
|
Storage |
-20˚C for two years |
|
Shipping Condition |
Dry ice (-70˚C) |
Kit Contents
| Component | JX101-01 | JX101-02 |
| FlyCut® HindIII | 1,500 units | 2 × 1,500 units |
| 10× FlyCut® Buffer | 500 μl | 1 ml |
| 10× DNA Loading Buffer | 1 ml | 1 ml |
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