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2×EasyTaq® PCR SuperMix ( +dye )

EasyTaq® PCR SuperMix is a ready-to-use mixture of EasyTaq DNA Polymerase, dNTPs, and optimized reaction buffer at a concentration of 2x. For DNA amplification, simply add the template, primers, and water to allow the SuperMix solution to react at a concentration of 1×. Please note, PCR products using this enzyme are not suitable for polyacrylamide gel electrophoriesis (PAGE).

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Highlights

• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 4 kb.

Application

Routine PCR

Storage

-20°C for two years

 

Kit Contents

Component

AS111-11

AS111-12

AS111-13

2×EasyTaq® PCR SuperMix(-dye) / (+dye)

1 ml

5 × 1 ml

15 × 1 ml

ddH2O

1 ml

5 ml

3 × 5 ml

 

Reaction System

Component

Volume

Final Concentration

Template

Variable

As required

Forward Primer (10 μM)

1 μl

0.2 μM

Reverse Primer (10 μM)

1 μl

0.2 μM

2×EasyTaq® PCR SuperMix

25 μl

ddH2O

Variable

-

Total volume

50 μl

-