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TransLv™ Lentivirus qPCR Titration Kit TransLv™ Lentivirus qPCR Titration Kit

TransLv™ Lentivirus qPCR Titration Kit

TransLv™ Lentivirus qPCR Titration Kit provides a simple, rapid, sensitive and efficient method to determine the copy number of integrated proviral sequences in lentivirus transduced cells using qPCR method. Lentivirus titer can be calculated from integrated proviral copy number in the genome of the lentivirus transduction susceptible cells (such as HT1080, HEK-293T, etc.) by using the standard curve generated from qPCR.

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TransLvTM Lentivirus qPCR Titration Kit provides a simple, rapid, sensitive and efficient method to determine the copy number of integrated proviral sequences in lentivirus transduced cells using qPCR method. Lentivirus titer can be calculated from integrated proviral copy number in the genome of the lentivirus transduction susceptible cells (such as HT1080, HEK-293T, etc.) by using the standard curve generated from qPCR.
 

• Simple operation and high sensitivity.
• Higher accuracy enabled by internal reference gene.
• Compatible with the second and third generation HIV-1 lentiviral packaging vectors.
• Good linear relationship in the range of 103-108 copies/μl.
 

Storage
at -20°C in the dark for one year.
 

Shipping
Dry ice (-70℃)

Product Contents

 

Component

FV201-01 (100 rxns)

TransLvTM Lentivirus qPCR Titration SuperMix

1 ml

10×GC enhancer

200 μl

Provirus Gene Standards (P1-P6) (108-103 copies/μl)

30 μl each

Reference Gene Standards (R1-R6) (108-103 copies/μl)

30 μl each

Provirus Gene Primer Mix (5 μM)

100 μl

Reference Gene Primer Mix (5 μM)

100 μl

Passive Reference Dye(50×)

40 μl

Nuclease-free Water

1 ml