FlyCut® BamHI is expressed and purified from E.coli that carries the recombinant BamHI gene. The molecular weight is 27.5 kDa, with the recognition site at G^GATCC. The reaction is conducted for 5-10 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.
Recognition Site
5’...G↓GATCC...3’
3’...CCTAG↑G...5’
Applications
Genomic DNA, plasmid DNA, PCR product
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
Product Contents
|
Component |
JB101-01 |
JB101-02 |
|
FlyCut® BamHI |
5,000 units |
2×5,000 units |
|
10×FlyCut® Buffer |
2×1 ml |
3×1 ml |
|
10×DNA Loading Buffer |
1 ml |
1 ml |
Citations
1.Lu C , Ma J , Cai D . Increased HAGLR expression promotes non–small cell lung cancer proliferation and invasion via enhanced de novo lipogenesis[J]. Tumor Biology, 2017, 39(4):101042831769757.
2.Jian‑Zhi Gao, Li J , Jing‑Li Du, et al. Long non-coding RNA HOTAIR is a marker for hepatocellular carcinoma progression and tumor recurrence[J]. Oncology letters, 2016, 11(3):1791-1798.
US
Europe (English)
Chinese
Japanese