Highlights |
• Extension rate is about 1-2 kb/min. |
Applications |
• Routine PCR |
Unit Definition |
One unit of EasyTaq® DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C. |
Quality Control |
EasyTaq® DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch of EasyTaq® DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA. |
Storage Buffer |
20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers. |
Storage |
-20°C for two years |
Shipping Condition |
Dry ice (-70°C) |
Kit Contents
Component |
AP111-11 |
AP111-12 |
AP111-13 |
EasyTaq® DNA Polymerase |
500 U×1 |
500 U×6 |
2500 U × 4 |
10× EasyTaq® Buffer |
1.2 ml×1 |
1.2 ml×6 |
1.2 ml × 20 |
2.5 mM dNTPs |
- / 800 μl×1 |
- / 800 μl ×6 |
- / 800 μl × 20 |
6× DNA Loading Buffer |
1 ml×1 |
1 ml×2 |
1 ml ×4 |