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EasyTaq® DNA Polymerase (with 2.5 mM dNTPs)

EasyTaq® DNA Polymerase is isolated from E. coli expressing a cloned DNA polymerase gene from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq DNA Polymerase has 5'-->3' DNA polymerase and exonuclease activity and is suitable for routine polymerase chain reaction (PCR) applications. Please note, PCR products using this enzyme are not suitable for polyacrylamide gel electrophoresis (PAGE).

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Highlights

• Extension rate is about 1-2 kb/min.
•Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY® -T vectors.
• Amplification of genomic DNA fragment up to 4 kb.

Applications

• Routine PCR
• Colony PCR

Unit Definition

One unit of EasyTaq® DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C.

Quality Control

EasyTaq® DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch of EasyTaq® DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

Storage Buffer

20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers.

Storage

-20°C for two years 

Shipping Condition

Dry ice (-70°C)

 

 

Kit Contents

Component

AP111-11

AP111-12

AP111-13

EasyTaq® DNA Polymerase

500 U×1

500 U×6

2500 U × 4

10× EasyTaq® Buffer

1.2 ml×1

1.2 ml×6

1.2 ml × 20

2.5 mM dNTPs

- / 800 μl×1

- / 800 μl ×6

- / 800 μl × 20

6× DNA Loading Buffer

1 ml×1

1 ml×2

1 ml ×4