EasyPure® Plasmid MiniPrep Kit uses a modified alkaline lysis method to isolate high-quality plasmid DNA from ≤20 ml (LB) of bacterial culture. Unique formulated lysis buffer and neutralization buffer permit complete bacterial cell lysis and neutralization. The purified plasmid DNA is suitable for a variety of molecular biology applications, including restriction enzyme digestion, ligation, transformation and DNA sequencing.
• Simple and fast: the whole procedure can be performed in 20 minutes.
• High yield: DNA yield up to 40 μg.
Storage
RNase A at -20°C for two years; others at room temperature (15-25°C) in a dry place for one year.
Shipping
RNase A on dry ice (-70 °C); others at room temperature.
Product Contents
|
Component |
EM101-01 (50 rxns) |
EM101-02 (200 rxns) |
|
Resuspension Buffer (RB) |
15 ml |
60 ml |
|
Lysis Buffer (LB) |
15 ml |
60 ml |
|
Neutralization Buffer (NB) |
20 ml |
80 ml |
|
Wash Buffer (WB) |
10 ml |
2×20 ml |
|
Elution Buffer (EB) |
5 ml |
10 ml |
|
RNase A (10 mg/ml) |
150 μl |
600 μl |
|
Mini-Plasmid Spin Column with Collection Tubes |
50 each |
2×100 each |
Citations
Xiao C C , Zhang J , Luo P C , et al. Identification of Tisp40 as an Essential Regulator of Renal Tubulointerstitial Fibrosis via TGF-β/Smads Pathway[J]. Cellular Physiology and Biochemistry, 2017:697-712.
Luo J , Yang J , He H , et al. A new electrochemically active bacterium phylogenetically related to Tolumonas osonensis and power performance in MFCs[J]. Bioresource Technology, 2013, 139:141-148.
Li X K , Chu Z R , Liu Y J , et al. Molecular characterization of microbial populations in full-scale biofilters treating iron, manganese and ammonia containing groundwater in Harbin, China[J]. Bioresource Technology, 2013, 147(Complete):234-239.
Wang B , Xiao J H , Bian S N , et al. Evolution and Expression Plasticity of Opsin Genes in a Fig Pollinator, Ceratosolen solmsi[J]. PLOS ONE, 2013, 8(1):e53907.
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