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TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix

Unique genomic DNA remover is combined with TransScript® First-Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds.

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Highlights

• Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• cDNA up to 12 kb.

Applications

Multiple copy and low copy gene detection

Storage

-20˚C for two years

Shipping Condition

Dry ice (-70˚C)

 

Kit Contents

Component

AT311-02 (50 rxns)

AT311-03 (100 rxns)

TransScript® RT/RI Enzyme Mix

50 μl

100 μl

gDNA Remover

50 μl

100 μl

2× TS Reaction Mix

500 μl

1 ml

Random Primer (N9) (0.1 μg/μl)

50 μl

100 μl

Anchored Oligo(dT) 18 Primer (0.5 μg/μl)

50 μl

100 μl

RNase-free Water

500 μl

1 ml