GENEWIZ | Genomic DNA Extraction Kit

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TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix

Unique genomic DNA remover is combined with TransScript® First-Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds.

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50rxns×20µlSystems
100rxns×20µlSystems

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ProductIntroduction
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Highlights	• Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination. • The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR. • cDNA up to 12 kb.
Applications	Multiple copy and low copy gene detection
Storage	-20˚C for two years
Shipping Condition	Dry ice (-70˚C)

Kit Contents

Component	AT311-02 (50 rxns)	AT311-03 (100 rxns)
TransScript® RT/RI Enzyme Mix	50 μl	100 μl
gDNA Remover	50 μl	100 μl
2× TS Reaction Mix	500 μl	1 ml
Random Primer (N9) (0.1 μg/μl)	50 μl	100 μl
Anchored Oligo(dT) 18 Primer (0.5 μg/μl)	50 μl	100 μl
RNase-free Water	500 μl	1 ml

TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix