Unique genomic DNA remover is combined with TransScript® II First- Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis at 42-55°C. After cDNA synthesis, gDNA remover and TransScript® II RT/RI Enzyme Mix are inactivated by heating at 85°C for 5 seconds.
• Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• After reaction, heat inactivation for RT/RI and gDNA remover simultaneously can avoid damage to RNA caused by heat inactivating DNase I used for pretreatment in traditional protocol.
• Simple and fast procedure.
• cDNA up to 15 kb.
Applications
• cDNA library construction, 3’ and 5’ RACE
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
Product Contents
|
Component |
AH311-02 (50 rxns) |
AH311-03 (100 rxns) |
|
TransScript® II RT/RI Enzyme Mix |
50 μl |
100 μl |
|
gDNA Remover |
50 μl |
100 μl |
|
2×TS II Reaction Mix |
500 μl |
1 ml |
|
Random Primer (0.1 μg/μl) |
50 μl |
100 μl |
|
Anchored Oligo(dT)20 Primer (0.5 μg/μl) |
50 μl |
100 μl |
|
RNase-free Water |
500 μl |
1 ml |
Citations
Li Y , Deng H , Miao M , et al. Tomato MBD5, a methyl CpG binding domain protein, physically interacting with UV-damaged DNA binding protein-1, functions in multiple processes[J]. New Phytologist, 2016, 210(1):208-226.
Zeng J , Ding Q , Fukuda H , et al. Fertilization Independent Endosperm genes repress NbGH3.6 and regulate the auxin level during shoot development in Nicotiana benthamiana[J]. Journal of Experimental Botany, 2016, 67(8):erw024.
Zou X , Qin Z , Zhang C , et al. Over-expression of an S-domain receptor-like kinase extracellular domain improves panicle architecture and grain yield in rice[J]. Journal of Experimental Botany, 2015, 66(22):7197-7209.
Wang F , Lin R , Feng J , et al. TaNAC1 acts as a negative regulator of stripe rust resistance in wheat, enhances susceptibility to Pseudomonas syringae, and promotes lateral root development in transgenic Arabidopsis thaliana[J]. Frontiers in Plant Science, 2015, 27(6):108.
Li Q , Wang Y , Wang F , et al. Functional conservation and diversification of APETALA1/FRUITFULL genes in Brachypodium distachyon[J]. Physiologia Plantarum, 2016, 157(4):507-518.
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