# of Aliquots: The table below shows how we aliquot your sample volumes into tubes. If you would like your sample aliquoted in any other way, by volume or yield, that can be done for you at an additional charge. Examples for custom aliquot requests are also listed.
|Run Scale||Sample Volumes|
|Mini||60μl mini tube or 96-well plate depending on order size|
|Midi||200μl in 2ml tube|
|Maxi||500μl in 2ml|
|Mega||1ml in 2ml tube|
|Giga||(1ml in 2ml tube) x2|
|Normalizations||1.7ml per tube 2ml (leaving room for expansion if freezing) or mini tubes (<200ul)|
Example 1: 50μl in one tube, the rest in a second tube.
Example 2: Split the order into 5 tubes.
Example 3: 50μg per tube.
Custom Tubes: If you would like your samples shipped back to you in specific tubes from your stock, that can be done for you at an additional charge. Simply include a stock of your tubes with your samples.
Fresher is better; < 3days old with well isolated colonies. Older plates can be freshly streaked for isolation upon request. Please note that this will add to the order timeline.
Please use caution in shipping agar plates during the cold winter months. The agar media may freeze in transit, rendering the plate unusable. Glycerol stocks are recommended as a substitute format during this season.
Larger plasmids (>10 kb) and plasmids containing toxic or unstable sequences (such as long terminal repeats (LTR) found in viral expression vectors) are likely to produce better results when paired with cell lines engineered to maintain these difficult sequences.
We advise against using standard cloning strains for these plasmids, due to increased risk of lower yield and plasmid instability. We also suggest the selection of additional QC testing to verify unstable or toxic plasmids.
Our internal quality monitoring will trigger the automatic repeat of a plasmid DNA preparation when the yield is below a critical threshold, depending on the anticipated copy number for the plasmid. Yield variance above this threshold could be due to a variety of factors and may be intrinsic to the sample provided.
Plasmid DNA preparation orders are processed by run. For low copy or other low yield plasmids, we recommend ordering a larger preparation scale or multiple preps, depending on the amount of final material required.
To discuss troubleshooting or custom process modifications, please contact Azenta Technical Support.