MagicPure® Viral DNA/RNA Kit utilizes a unique lysis buffer to lyse virus and release DNA / RNA. The released DNA / RNA is effectively purified after specifically binding to silica magnetic beads. It is suitable for isolating viral DNA/RNA from up to 200 μl of plasma, serum, whole blood, tissue homogenate, cell-free body fluid, nasopharyngeal or oropharyngeal aspirate/ wash, bronchoalveolar lavage fluid (BALF), tracheal aspirate, sputum, nasopharyngeal or oropharyngeal swab and animal cell culture supernatant. The isolated DNA/RNA with high purity can be applied in PCR, RT-PCR, qPCR, and qRT-PCR, etc. This kit is compatible with high-throughput magnetic-rod nucleic acid extractor.
• Simple and fast, no centrifugation required.
• High quality, free of contaminants and inhibitors.
Proteinase K solution at -20°C for two years; Magnetic Virus Beads at 2-8°C for one year (protect from freezing); others at room temperature (15°C-25°C) for one year.
Proteinase K on dry ice (-70 °C); Magnetic Virus Beads on Ice bag (4 °C); others at room temperature.
|Component||EC301-01/11 (50 rxns)|
|Binding Buffer 19(BB19)||18 ml|
|Clean Buffer 19(CB19)||18 ml|
|Wash Buffer 19(WB19)||12 ml|
|RNase-free Water||10 ml|
|Proteinase K (20 mg/ml)||1 ml|
|Magnetic Virus Beads||1 ml|
|Magnetic Stand(16 hole)||1 each/-|
1.Xie L , Huang L , Xie Z , et al. An attempt to develop a detection method specific for virulent duck plague virus strains based on the UL2 gene B fragment[J]. Avian Pathology, 2017:1-17.