DNA methylation is an epigenetic modification that alters gene expression, often as a repressor. In eukaryotes, the nucleotide cytosine is commonly methylated to form 5-methylcytosine (5mC), especially when accompanied by guanine (CpG). DNA methylation occurs in both animals and plants, playing significant roles in embryonic development, tissue regeneration, and pathogenesis.
To uncover the methylome, Azenta Life Sciences, formerly GENEWIZ offers wet and dry lab solutions, including Methyl-Seq, for global whole genome and targeted 5-methylcytosine (5mC) detection.
Submit cells, tissue, or gDNA for enzymatic conversion of unprotected cytosine
Add adapters to ssDNA fragments
Sequence 2x150 bp on Illumina® platform
Identify 5mC sites, calculate methylation ratios, and perform differential methylation analysis
After protecting modified cytosines from downstream deamination, an enzymatic step deaminates cytosine but does not affect 5caC and 5ghmC. The resulting converted sequence can be used to compare treated and untreated samples, creating a snapshot of 5mC methylation patterns across the whole genome or targeted regions of interest.
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