Quantitative Polymerase Chain Reaction (qPCR)

 

Quantitative PCR (also known as real-time PCR or qPCR) is a commonly used tool for detecting, characterizing, and quantifying nucleic acids for a variety of applications in genomics, drug development, diagnostics, and biologics testing. Azenta's end-to-end, streamlined workflows for qPCR services include the best quality lab procedures, fast processing times, and expert consultations to bring our customers the most value.


   

NGS, qPCR, or Sanger Sequencing:
An Assay Selection Guide

qPCR Applications

Confirmatory testing for NGS data
Copy Number Variation (CNV) analysis
Genotyping and allelic discrimination
Gene expression assays and SNP genotyping
Nucleic acid and CpG methylation analysis 
Pathogen detection and quantification

qPCR Workflow

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    1. qPCR Assay/Primer Design

  • 2. Sample Preparation

  • 3. qPCR Processing

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    4. Data Analysis & Reporting

Features & Benefits

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    Flexible Workflows for any input material 

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    Custom options for primer design

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    Integrate qPCR with other Azenta Life Sciences services

Expertise in Assay Development - Our highly-trained team members are experts in primer design, optimization, and troubleshooting.
Streamlined End-to-End Workflow - GENEWIZ experts handle the complete workflow from sample preparation to final analysis.
One-Stop Shop - Seamless transition from GENEWIZ RUO-, GLP-, and CLIA-level upstream and downstream application into qPCR services.
High-Throughput Capacity - Scalable qPCR solutions to rapidly scale up pilot or exploratory projects.

Choice of Material


Cell or Tissue

Visualize results through interactive software and receive custom analysis 

 


DNA

Visualize results through interactive software and receive custom analysis 


RNA or cDNA

Visualize results through interactive software and receive custom analysis 

Deliverables

Relative Quantification

Measure gene expression, confirm NGS data, quantify copy numbers, and more

 

Genotyping

Characterize SNPs and detect heterozygotes for a variety of sample types

Custom Analysis

Choose from custom analyses or raw data delivery

Blog | NGS, qPCR, or Sanger Sequencing: An Assay Selection Guide

This selection guide offers practical information about PCR + Sanger, qPCR, and NGS approaches to help you determine which assay best suits your project requirements, along with an interactive assay selection tool to aid your decision making.

HOW TO ORDER


Email | Phone 1-877- 436-3949, Ext. 1