Sample Submission Guidelines
For the best Sanger sequencing results, Azenta Life Sciences, formerly GENEWIZ, strongly recommends you follow our Sample Submission Guidelines as closely as possible. If you have any questions, our Technical Support team is here to help!
(EU) Email: sanger.europe@azenta.com | Phone +49 (0)341 520 122-41
(UK) Email: genomics.service.uk@azenta.com | Phone +44 (0) 1279 873837
• PCR Products - Submit 10-40 ng per kb of amplicon.
• ITRs - For plasmids and PCR products that contain ITRs, please submit an additional 1 µg of material per ITR in the sample sequence.
• Primers - GENEWIZ can synthesize the initial primer if the sequence for a known region (e.g. antibiotic resistance) is provided. If you have primers that can be used in the initial round of sequencing, please submit a minimum of 10 µl each at 5 µM.
• Packaging - For DNA and primers, please use 1.5 ml screw cap tubes or microcentrifuge tubes secured with parafilm and label them with sample or primer name and concentration.
• Plasmid - Submit 1 µg per kb of target sequence for single-stranded coverage, 2 µg per kb sequence for double-stranded coverage, and 4 µg per kb for 4x coverage. Please submit a minimum concentration of 200 ng/µl per sample.
• PCR Products - Submit 10-40 ng per kb of amplicon.
• ITRs - For plasmids and PCR products that contain ITRs, please submit an additional 1 µg of material per ITR in the sample sequencing.
• Primers - GENEWIZ can synthesize all of the primers if the known reference sequence is provided. If you have primers that can be used in the initial round of sequencing, please submit a minimum of 10 µl each at 5 µM.
• Packaging - For DNA and primers, please use 1.5 ml screw cap tubes and microcentrifuge tubes secured with parafilm and label them with sample or primer name and concentration.
EU - Send samples to GENEWIZ EU via standard overnight service
Ship to:
GENEWIZ Germany GmbH
Bahnhofstrasse 86
04158 Leipzig
Germany
Or check out our EU shipping logistics options
UK -Send samples to GENEWIZ UK via standard overnight service
Ship to:
GENEWIZ UK LTD
Little Bullock Farm
Hope End
Takeley
Essex
CM22 6TA
UK
Or check out our UK shipping logistics options