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DNA Sequencing Sample Submission Guidelines


For the best Sanger sequencing results, GENEWIZ strongly recommends you follow our Sample Submission Guidelines as closely as possible. If you have any questions, our Technical Support team is here to help! 

(EU) Email: sanger.europe@azenta.com | Phone +49 (0)341 520 122-41

(UK) Email: genomics.service.uk@azenta.com | Phone +44 (0) 1279 873837


Service options

Primer Walking: Sequence Discovery

• Plasmid - Submit 1 µg per kb of target sequence for single-stranded coverage, 2 µg per kb sequence for double-stranded coverage, and 4 µg per kb for 4x coverage. Submit at least 10 µg plasmid if region of interest is of unknown length. Please submit a minimum concentration of 200 ng/I per sample.


• PCR Products 
- Submit 10-40 ng per kb of amplicon.

• ITRs - For plasmids and PCR products that contain ITRs, please submit an additional 1 µg of material per ITR in the sample sequence.

• Primers - GENEWIZ can synthesize the initial primer if the sequence for a known region (e.g. antibiotic resistance) is provided. If you have primers that can be used in the initial round of sequencing, please submit a minimum of 10 µl each at 5 µM.

• Packaging - For DNA and primers, please use 1.5 ml screw cap tubes or microcentrifuge tubes secured with parafilm and label them with sample or primer name and concentration.

 

Primer Walking: Sequence Confirmation

• Plasmid - Submit 1 µg per kb of target sequence for single-stranded coverage, 2 µg per kb sequence for double-stranded coverage, and 4 µg per kb for 4x coverage. Please submit a minimum concentration of 200 ng/µl per sample.

• PCR Products - Submit 10-40 ng per kb of amplicon.

• ITRs - For plasmids and PCR products that contain ITRs, please submit an additional 1 µg of material per ITR in the sample sequencing.

• Primers - GENEWIZ can synthesize all of the primers if the known reference sequence is provided. If you have primers that can be used in the initial round of sequencing, please submit a minimum of 10 µl each at 5 µM.

• Packaging - For DNA and primers, please use 1.5 ml screw cap tubes and microcentrifuge tubes secured with parafilm and label them with sample or primer name and concentration.

 

Shipping

EU - Send samples to GENEWIZ EU via standard overnight service

Ship to:
GENEWIZ Germany GmbH
Bahnhofstrasse 86
04158 Leipzig
Germany

Or check out our EU shipping logistics options

 

UK -Send samples to GENEWIZ UK via standard overnight service

Ship to:
GENEWIZ UK LTD
Little Bullock Farm
Hope End
Takeley
Essex
CM22 6TA
UK

Or check out our UK shipping logistics options