GENEWIZ’s new proprietary Sanger sequencing method sequences through difficult inverted terminal repeat (ITR) regions of adeno-associated virus (AAV), to expedite screening and validation of leads for your cell and gene therapy research. 

This newly-developed protocol prevents abrupt reduction in the sequencing signal at the start of the ITR hairpin and reads through the full length of the ITR region. AAV-ITR sequencing can be combined with our Primer Walking service to sequence whole AAV vectors.




GENEWIZ GLP-Compliant AAV-ITR Sanger Sequencing

The first and only available GLP-compliant method for sequencing through difficult ITR regions of AAV vectors

GENEWIZ AAV-ITR Sequencing Provides a Breakthrough Solution

Adeno-associated virus (AAV) is one of the most actively investigated gene therapy vehicles. GENEWIZ’s robust AAV-ITR Sequencing Protocol sequences through its difficult inverted terminal repeat (ITR) regions to better support your viral vector testing and drug discovery pipeline.

Standard Protocol


AAV-ITR Sequencing – GENEWIZ Proprietary Protocol



Qualitative assessment of the integrity of wild-type and partially-truncated ITR regions in AAV plasmids 
Ph.D.-level customer support throughout the project
Turnaround time starting at just 5 business days
Increased read lengths and improved data quality for early detection of point mutations
Advanced network of dropboxes and couriers for convenient sample submission
Online ordering system allows real-time order management
Webinar: Effective and High-Quality Solutions for AAV-Based Therapies

Webinar: Effective and High-Quality Solutions for AAV-Based Therapies


In this webinar, GENEWIZ’s Dr. Eric Zhao will present our unique AAV-ITR Sanger sequencing and AAV plasmid preparation capabilities that maintain ITR integrity for downstream virus production, as well as our next generation sequencing-enabled QC of packaged rAAV vectors and host genomes using long-read and short-read sequencing technologies.

AAV ITR Sequencing Case Study

Tech Note: Reading Through the Inverted Terminal Repeats (ITRs) of Adeno-associated Virus (AAV)


GENEWIZ has developed a high-quality, direct Sanger sequencing method that reads through both intact and commonly mutated inverted terminal repeat (ITR) regions of adeno-associated virus (AAV). This method is an effective tool for assessing the integrity of ITRs in AAV plasmids.


*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service.  Note that direct-sequencing templates are not available for our Same Day service.

Email | Phone (1-877-GENEWIZ (436-3949), Ext. 3350)