Whole Genome Sequencing

Whole genome sequencing (WGS) provides unprecedented access to genomic information and promises to deliver breakthroughs in personalized medicine, pharmacogenomics, agrigenomics, oncogenomics, phylogenetics, and biomarker discovery.

Now with the PacBio Sequel and Illumina® HiSeq™ X Ten!

At GENEWIZ, we are using next generation sequencing platforms like the powerful Illumina® HiSeq™ X Ten and the long-read PacBio Sequel to support researchers all over the world with their genome sequencing needs.  Our capabilities include;

  • Germline variant detection
  • Somatic variant detection
  • de novo genome assembly
  • Whole genome re-sequencing
  • Structural variant discovery
  • Copy number variation

While the greatest interest for whole genome sequencing tends to be related to human genomes, GENEWIZ supports whole genome analysis of any species including plants, animals, bacteria, and viruses.


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Building

Case Study

Detection of Previously Unidentified Structural Variants
Involved in Glioblastoma Using PacBio Sequel Sequencing

Whole Genome Sequencing on the PacBio Sequel

Whole Genome Sequencing with the PacBio Sequel utilizes Single Molecule, Real-Time (SMRT) technology that combines long read technology and rapid sequencing to produce the highest-quality de novo assemblies, phase alleles and variants, and significantly improve the detection of large structural variants. The new PacBio Sequel system uses SMRT cells containing 1 million Zero-Mode Waveguides to generate 7x the output of the PacBio RSII with up to 99.999% accuracy.



Long Reads Span Most Variants

Whereas short read technologies are limited in their ability to identify Indels, a single PacBio read can discover a wide range of variants, from SNPs and small Indels to large insertions and deletions. Assembled, PacBio reads even allow for identification of large structural rearrangements and phasing of variants, even in highly repetitive sequences.

Long Reads PacBio


Applications

Generate complete de novo assemblies for small/medium sized genomes
Phase alleles and variants
Detect DNA modifications without bisulfite treatment
Obtain high quality de novo assemblies of large and complex genomes
Identify large structural variants

Features & Benefits

7X the data output of the PacBio RSII
Up to 60 kb continuous reads
No PCR amplification bias
Up to 99.999% accuracy
10-15 kb average read length

Whole Genome Sequencing on the Illumina HiSeq X Ten

Whole genome sequencing with the Illumina HiSeq X Ten utilizes Sequencing by Synthesis (SBS) technology that delivers unmatched throughput power ideal for large-scale whole genome sequencing projects. The platform’s unprecedented price point and speed provides scientists the opportunity to expand into all the key research market segments – human, livestock, plants, and model or medically relevant organisms – for their whole genome sequencing needs.

Illumina HiSeq X Ten

Applications

Whole genome re-sequencing
de novo whole genome assembly
Germline variant detection
Somatic variant detection
Copy number variation

Features & Benefits



Maximum throughput platform

Integrated end-to-end solutions

Low input starting material requirements accepted

Highest coverage at lowest cost

Human and non-human species

Comprehensive suite of bioinformatics solutions; publication-grade data

Whole Genome Sequencing Workflow

Extensive Quality Control is Performed at the Conclusion of Each Step of the Process

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    Experimental
    Design



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    Library
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    Sequencing



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    Bioinformatics
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Deliverables

All customers receive their raw data as FASTQ files. Customizable data analysis packages are available by request.


NGS Platforms

GENEWIZ is a certified service provider for Illumina and Life Technology NGS platforms. For information on our NGS platforms as well as recommended configurations for your projects, please visit the NGS Platforms page. GENEWIZ does not guarantee data output or quality for sequencing only projects.

 


How To Order


Email | Phone (1-877-GENEWIZ (436-3949), Ext. 1) | Live Chat