Whole Genome Sequencing
Whole genome sequencing (WGS) is the comprehensive reading and analysis of an entire genome, including intronic, exonic, and intergenic regions. It is increasingly used in genome wide association studies (GWAS) for higher resolution and rare variant detection to enable scientists to understand the genetic roots of complex conditions and understand population evolution.
GENEWIZ Whole Genome Sequencing services use cutting-edge technologies and bioinformatics to deliver high-quality data for human, animal, plant, bacterial, or viral genomes, with flexible options for read length and coverage to meet your research needs.
GENEWIZ Whole Genome Sequencing
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Expert DNA extraction services available, with over 30 standard types and hundreds of custom sample types
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Flexible library preparation options, including PCR-free, low-input, cell-free DNA (cfDNA) and circulating tumor DNA (ctDNA)
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Choice of sequencing coverage and platform, including Illumina NovaSeq X Plus and MiSeq, PacBio® Revio® and Oxford Nanopore Technologies® PromethION® / GridION®
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Flexible data amounts, starting at 1Gb + read pairs
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Available in Research Use Only, Regulated and CLIA service-levels
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U.S.-Based Processing – Sequencing and customer support in New Jersey
Whole Genome Sequencing Services
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Short-read Human Whole Genome Sequencing
- Illumina standard 2x150bp configuration.
- Easy-to-order bundled prep (with PCR or PCR-free) and sequencing options for 90-300+ Gb per sample.
- CLIA-compliant Whole Genome Sequencing available.
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Short-read Plant and Animal Whole Genome Sequencing
- Illumina standard 2x150bp configuration.
- Easy-to-order bundled prep and sequencing options for 1-300+ Gb per sample.
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Short-read Bacterial Whole Genome Sequencing
- Illumina 2x150bp, 2x250bp or 2x300bp configuration.
- Easy-to-order bundled prep and sequencing options with 1 – 6+ Gb per sample.
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Low-Pass Whole Genome Sequencing
- Affordable alternative to genotyping arrays for rare variant discovery with greater power and richer data.
- Up to 99% accuracy of variant call detection at a very low sequencing coverage (0.1X – 10X) using imputation algorithms.
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Long-Read Whole Genome Sequencing
- Long-read sequencing on PacBio® or Oxford Nanopore Technologies® platforms of up to 100kb reads.
- CLIA-compliant whole genome sequencing available.
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Clinical Long- and Short-read Human Whole Genome Sequencing
- For clinical research and translational studies.
- Analytically and clinically-validated CLIA-compliant whole genome sequencing available.
Whole Genome Sequencing Applications and Technology
Illumina short-read sequencing is ideal for high-throughput, cost-effective studies requiring accurate variant detection across diverse organisms, even from low-input samples. It is best suited for applications like SNP detection and population genomics.
Long-read sequencing with PacBio (up to 25 kb) or Oxford Nanopore (up to 4 Mb) spans large repeats, making it ideal for complex genomes and structural variant detection. PacBio excels at de novo genome assembly, while ONT is best for rapid pathogen identification.
| Technology | Application | Read Depth |
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Short-read whole genome sequencing from Illumina |
Genotyping, GWAS | 5-10x |
| Germline/frequency variant analysis, Population studies, tumor vs normal | 20-50x | |
| Somatic/rare variants, de novo assembly | 100-1000x | |
Long-read whole genome sequencing PacBio and Oxford Nanopore |
Gap filling and scaffolding, Large structural variant detection | 10x |
| Germline/frequency variant analysis | 20-50x | |
| De novo assembly | 30-100x |
Please note suggested read depth may vary depending on organism/project goals.
GENEWIZ Whole Genome Sequencing Workflow
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1. Submit Samples
Easy ordering and sample submission via dropbox or direct shipping
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2. Extract
High-quality extraction available from blood, tissue, FFPE, saliva, and more—plus dual DNA/RNA and cell free DNA options
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3. Library Prep
Scalable library prep with PCR-Free, amplification, low-pass and whole genome amplification for low- input samples
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4. Sequence
Sequencing on Illumina, PacBio® and Oxford Nanopore Technologies® platforms
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5. Analyze Data
Mapping, SNP/indel detection, SVs, CNVs and more. Comprehensive analysis includes intronic, exonic, and intergenic regions
Technical Resources
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Quick Start Guide | Exploring Bioinformatics for Genome and Transcriptome Sequencing Data
For those new to bioinformatics, analyzing massive amounts of NGS data can be a daunting task. Download GENEWIZ’s bioinformatics quick start guide to learn how to analyze whole genome sequencing (WGS) and RNA sequencing (RNA-Seq) data with bioinformatics tools to reveal biological insights for your research.
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Webinar | A New Paradigm in DNA Sequencing – Highly Accurate Single-Molecule Long Reads (Hosted by PacBio)
In this PacBio-hosted webinar, Jonas Korlach, PacBio Chief Scientific Officer, and Dave Corney, GENEWIZ Associate Principal Scientist, Next Generation Sequencing, describe the recent release of Sequel System 6.0, which has revolutionized long-read sequencing by providing users the ability to generate highly accurate single-molecule reads.
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Best Practices | Preparation of High Molecular Weight DNA
High-quality high molecular weight (HMW) genomic DNA (≥50 kb) is critical to achieving long read lengths on platforms such as the 10x Genomics® Chromium™ and the PacBio Sequel. Follow these guidelines to generate the best possible WGS results.
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Webinar | Nanopore Results & Interpretation Made EZ
Nanopore sequencing provides a cost-effective, long-read approach for a variety of analyses, including whole plasmid sequencing and amplicon sequencing. This workshop looks at the considerations of each technique and how to interpret resulting sequencing data effectively.
NGS Platforms
For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. GENEWIZ does not guarantee data output or quality for sequencing-only projects.
