CANCER PANELS

 

Cancer panels use next generation sequencing (NGS) technology to target specific genes or mutations that have established relevancy to a particular cancer phenotype. NGS facilitates sequencing of large genomic regions, high numbers of genes, and/or high numbers of samples in a single, efficient and cost-effective assay. In addition, NGS provides significantly higher sensitivity than traditional techniques, which permits the discovery of rare somatic mutations, many of which have been identified as important cancer drivers.

GENEWIZ offers a number of different cancer panels to provide a complete solution from experimental design to advanced bioinformatics analysis.

 

 

SERVICE OPTIONS

  • Building

    OncoGxOne™ Discovery Cancer Panels


    GENEWIZ OncoGxOne™ Discovery cancer panels provide comprehensive genomic analysis for cancer research, biomarker discovery, and patient stratification for clinical discovery. Designed and developed with the most recent research data available, these assays provide thorough assessments on cancer genes of interest.


  • Building

    Hotspot Cancer Panels


    Hotspot cancer panels target well-characterized, small regions of known cancer genes. The small target region size produces a high depth of sequencing coverage per sample, even when multiplexing many samples together. As a result, hotspot cancer panels provide a cost-effective and efficient method for discovering rare somatic mutations, many of which have been identified as important cancer drivers.


Features & Benefits


Extremely high depth of coverage to detect common and rare mutations

Very low input DNA requirement

Sequencing compatibility with FFPE samples

Capability to detect somatic point mutations, INDELs, gene fusions, and copy number variations (CNV)

Customizability of select cancer panels to add additional genes of interest

Dedicated support from experienced Ph.D. scientists

Complete solution, from project design through custom data analysis

Fast turnaround time with expedited options available

Cancer Panel Comparison


GENEWIZ OncoGxOneTM
Discovery Cancer Panels
Illumina TruSeq©
Amplicon Cancer Panel
Ion AmpliSeqTM
Cancer Hotspot Panel
Sequencing Platform Illumina MiSeq or HiSeq Illumina MiSeq Ion PGM®
Cancer Types Assayed 19 distinct gene panels, each assays a specific cancer (organ) type Single gene panel assays multiple cancer types Single gene panel assays multiple cancer types
Genes Covered ≈150 - 400 48 50
Genomic Variation Detection
  • Point Mutations
  • Indels
  • Rearrangements
  • Copy Number Variations (CNV)
  • Point Mutations
  • Indels
  • Point Mutations
  • Indels
Target Regions ≈ 2 - 5.4 Mb, consisting of all exons, UTRs, and relevant intron regions ≈35 Kb, consisting of mutational hot spots ≈ 39 Kb, consisting of mutational hot spots
Average Depth > 100x* > 100x* > 100x*
Target Enrichment Agilent SureSelect Targeted Enrichment Illumina Targeted Amplicon Sequencing Life Technologies Targeted Amplicon Sequencing
DNA Input > 100 ng > 250 ng > 10 ng
Sample Submission
  • FFPE thin sections (slides)
  • gDNA
  • Fresh, frozen samples
  • FFPE thin sections (slides)
  • gDNA
  • FFPE thin sections (slides)
  • gDNA
*Additional depth of coverage is available by request.

Sample Submission Guidelines

GENEWIZ accepts formalin-fixed, paraffin-embedded (FFPE) thin sections (slides), purified genomic DNA, or fresh, frozen samples for Cancer Panel projects. For detailed sample submission requirements please visit our Sample Submission Guidelines page.

Deliverables

All customers receive their raw data as FASTQ files. Mutation discovery repeats and customizable data analysis packages are available on request.

NGS Platforms - Now with ILLUMINA Novaseq

GENEWIZ is a certified service provider for Illumina NGS platforms and the PacBio Sequel. Please visit our NGS Platforms page for more information.



How To Order


Email | Phone (1-877-GENEWIZ (436-3949), Ext. 1) | Live Chat