CANCER PANELS

Cancer panels use next generation sequencing (NGS) technology to target specific genes or mutations that have established relevancy to a particular cancer phenotype. NGS facilitates sequencing of large genomic regions, high numbers of genes, and/or high numbers of samples in a single, efficient and cost-effective assay. In addition, NGS provides significantly higher sensitivity than traditional techniques, which permits the discovery of rare somatic mutations, many of which have been identified as important cancer drivers.

GENEWIZ offers a number of different cancer panels to provide a complete solution from experimental design to advanced bioinformatics analysis.

SERVICE OPTIONS

OncoGxOne™ Discovery Cancer Panels

GENEWIZ OncoGxOne™ Discovery cancer panels provide comprehensive genomic analysis for cancer research, biomarker discovery, and patient stratification for clinical discovery. Designed and developed with the most recent research data available, these assays provide thorough assessments on cancer genes of interest.
Hotspot Cancer Panels

Hotspot cancer panels target well-characterized, small regions of known cancer genes. The small target region size produces a high depth of sequencing coverage per sample, even when multiplexing many samples together. As a result, hotspot cancer panels provide a cost-effective and efficient method for discovering rare somatic mutations, many of which have been identified as important cancer drivers.

Features & Benefits

Extremely high depth of coverage to detect common and rare mutations

Very low input DNA requirement

Sequencing compatibility with FFPE samples

Capability to detect somatic point mutations, INDELs, gene fusions, and copy number variations (CNV)

Customizability of select cancer panels to add additional genes of interest

Dedicated support from experienced Ph.D. scientists

Complete solution, from project design through custom data analysis

Fast turnaround time with expedited options available

Cancer Panel Comparison

	GENEWIZ OncoGxOne^TM Discovery Cancer Panels	Illumina TruSeq© Amplicon Cancer Panel
Sequencing Platform	Illumina MiSeq or HiSeq	Illumina MiSeq
Cancer Types Assayed	19 distinct gene panels, each assays a specific cancer (organ) type	Single gene panel assays multiple cancer types
Genes Covered	≈150 - 400	48
Genomic Variation Detection	Point Mutations Indels Rearrangements Copy Number Variations (CNV)	Point Mutations Indels
Target Regions	≈ 2 - 5.4 Mb, consisting of all exons, UTRs, and relevant intron regions	≈35 Kb, consisting of mutational hot spots
Average Depth	> 100x*	> 100x*
Target Enrichment	Agilent SureSelect Targeted Enrichment	Illumina Targeted Amplicon Sequencing
DNA Input	> 100 ng	> 250 ng
Sample Submission	FFPE thin sections (slides) gDNA Fresh, frozen samples	FFPE thin sections (slides) gDNA

*Additional depth of coverage is available by request.

Sample Submission Guidelines

GENEWIZ accepts formalin-fixed, paraffin-embedded (FFPE) thin sections (slides), purified genomic DNA, or fresh, frozen samples for Cancer Panel projects. For detailed sample submission requirements please visit our Sample Submission Guidelines page.