While traditional, bulk assay for transposase-accessible chromatin sequencing (ATAC-Seq) offers valuable insights into genome-wide epigenetic regulation, cells in heterogeneous populations are combined into a single transposition reaction – obscuring key chromatin signatures. Utilizing microfluidics and molecular barcoding, single-cell ATAC-Seq (scATAC-Seq) investigates chromatin accessibility in hundreds to thousands of individual nuclei from a single sample, enabling regulatory motif discovery and offering a deeper understanding of gene regulation. For a single-cell multi-omic approach, scATAC-Seq and scRNA-Seq can be combined through an integrated data analysis framework to uncover novel biological features.
Azenta Life Sciences, formerly GENEWIZ's scATAC-Seq utilizes the latest technology on the market: the 10x Genomics® Chromium™ and Illumina® NovaSeq™. As an early adopter of these platforms, our optimized workflows — including pre-submission cryopreservation and post-submission dead cell removal — maximize project flexibility, speed, and data accuracy while eliminating costly overhead and ramp-up time to expedite your research.