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Next Generation Sequencing
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RNA-SeqHigh-Throughput Gene Expression Screening NEWSingle-Cell SequencingExome SequencingAmplicon Sequencing ServicesSequencing Only
CRISPR ValidationTargeted SequencingMetagenomicsEpigenomicsImmunogenomicsDigital Spatial ProfilingAAV Genome SequencingProtein Biomarker Detection with Olink NEW
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Plasmid DNA Preparation
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AAV Plasmid PreparationStandard Plasmid DNA PrepTargeted Yield Plasmid DNA Prep
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Sanger Sequencing
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AAV-ITR Sanger SequencingPurified TemplatesDifficult Template SequencingDirect Colony SequencingPCR PurificationPrimer Walking
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PCR + Sanger Services
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Bacterial and Fungal IdentificationMutation AnalysisSNP GenotypingPCR Plus Sequencing CRISPR Analysis PackageConfirmatory Sequencing & Cell Bank CharacterizationYeast Colony SequencingFragment AnalysisQuantitative Polymerase Chain Reaction (qPCR) NEW
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Gene Synthesis
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Standard Gene Synthesis (PriorityGENE)TurboGENE™Antibody DNA SynthesisAAV Plasmid SynthesisGene FragmentsCRISPR Construct SynthesisssDNA SynthesisDNA CloningSite-Directed Mutagenesis
Viral Packaging NEWRecombinant Antibody Production NEWCustom mRNA Synthesis NEW
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Oligo Synthesis Services
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Overnight DNA OligosCustom DNA and RNA OligosOligo Retained for Sanger Sequencing
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PreClinical and Clinical Services
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Clinical ServicesCLIA Sanger SequencingCLIA Whole Exome SequencingCLIA Whole Genome SequencingGLP-Compliant OverviewGLP Confirmatory SequencingGLP SNP Mutation AnalysisGLP Plasmid PrepGLP Nucleic Acid ExtractionBiofluid Processing
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Iso-Seq (Full-Length Isoform Sequencing)
Single Molecular, Real-Time (SMRT®) long-read technology, through PacBio Sequel, enables sequencing of contiguous full-length transcripts. This method, called Iso-Seq, avoids errors that can occur with short-read approaches, and captures each transcript’s start, polyadenylation, and splice sites from a single read.
Potential applications include better annotated genomes, detection of gene fusion, and discovery of novel isoforms.
The capabilities of Iso-Seq are greatly enhanced on the PacBio Sequel system compared to the older RSII. Azenta Life Sciences, formerly GENEWIZ has further optimized the process to exceed PacBio’s benchmarks in output and read length. Learn more here.
Contact our team to discuss how Iso-Seq on the new PacBio technology can improve your NGS projects.
TECHNICAL DETAILS
Isoform Sequencing on PacBio Sequel – Maximizing Output and Accuracy
Learn how Azenta has further optimized PacBio Sequel capabilities to exceed PacBio’s benchmarks in output and read length.
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Article: Which RNA-Seq Technique Should I Use?
With so many RNA sequencing assay types to choose from, how do you know which is best suited for your NGS project? In this article, we discuss the most common RNA-Seq approaches and what factors to consider when selecting the right one.
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Blog: PacBio HiFi Sequencing for Understanding Human Health and Disease
At the Azenta Life Sciences and PacBio virtual symposium titled, Decoding the Complexity of Human Health – A HiFi Vision, speakers from leading research institutions and biotech discussed the role of high-fidelity, long-read sequencing in understanding human health and diseases. In this blog post, we recap the presentations and highlight the themes that emerged from the virtual event.
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NGS Platforms
Azenta is a qualified service provider for Illumina and PacBio NGS platforms. For information on our NGS platforms as well as recommended configurations for your projects, please visit the NGS Platforms page.
Deliverables
All customers receive their raw data as BAM files. Additional data analysis including: annotation and full-length non-chimeric reads are available. Other custom reports are also available on request.
