Iso-Seq (Full-Length Isoform Sequencing)

 

Single Molecular, Real-Time (SMRT®) long-read technology, through PacBio Sequel, enables sequencing of contiguous full-length transcripts. This method, called Iso-Seq, avoids errors that can occur with short-read approaches, and captures each transcript’s start, polyadenylation, and splice sites from a single read.

Potential applications include better annotated genomes, detection of gene fusion, and discovery of novel isoforms.

The capabilities of Iso-Seq are greatly enhanced on the PacBio Sequel system compared to the older RSII. GENEWIZ has further optimized the process to exceed PacBio’s benchmarks in output and read length. Learn more here.

Contact our team to discuss how Iso-Seq on the new PacBio technology can improve your NGS projects.

 

TECHNICAL DETAILS

Isoform Sequencing on PacBio Sequel – Maximizing Output and Accuracy

Learn how GENEWIZ has further optimized PacBio Sequel capabilities to exceed PacBio’s benchmarks in output and read length.

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Features & Benefits

Optimized workflows deliver best output and accuracy when compared to PacBio benchmarks
Dedicated support from experienced GENEWIZ scientists
Lower input material requirements when compared to older technologies
Cost-effective with fast turnaround times

NGS Platforms

GENEWIZ is a qualified service provider for Illumina and PacBio NGS platforms. For information on our NGS platforms as well as recommended configurations for your projects, please visit the NGS Platforms page.

  



Sample Submission Guidelines

GENEWIZ accepts total RNA, mRNA, ds-cDNA and cell cultures/pellets and more for Iso-Seq projects. Please visit our Sample Submission Guidelines page for detailed requirements.

 

Deliverables

All customers receive their raw data as BAM files. Additional data analysis including: annotation and full-length non-chimeric reads are available. Other custom reports are also available on request.


How To Order


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