Single Molecular, Real-Time (SMRT®) long-read technology, through PacBio Sequel, enables sequencing of contiguous full-length transcripts. This method, called Iso-Seq, avoids errors that can occur with short-read approaches, and captures each transcript’s start, polyadenylation, and splice sites from a single read.
Potential applications include better annotated genomes, detection of gene fusion, and discovery of novel isoforms.
The capabilities of Iso-Seq are greatly enhanced on the PacBio Sequel system compared to the older RSII. Azenta Life Sciences, formerly GENEWIZ has further optimized the process to exceed PacBio’s benchmarks in output and read length. Learn more here.
Contact our team to discuss how Iso-Seq on the new PacBio technology can improve your NGS projects.
Azenta is a qualified service provider for Illumina and PacBio NGS platforms. For information on our NGS platforms as well as recommended configurations for your projects, please visit the NGS Platforms page.
All customers receive their raw data as BAM files. Additional data analysis including: annotation and full-length non-chimeric reads are available. Other custom reports are also available on request.