Isoform sequencing (Iso-Seq) is a type of RNA sequencing (RNA-Seq) method that uses Single Molecular, Real-Time (SMRT®) long-read technology to sequence contiguous, full-length transcripts. This method addresses the errors that can occur using short-read approaches and captures the transcript start, polyadenylation, and splice sites from a single read. Applications include better-annotated genomes, detection of gene fusion, and discovery of novel isoforms.

Instead of the RSII, our Iso-Seq service leverages the PacBio Sequel platforms to enhance Iso-Seq capabilities. Additionally, we have optimized the process to exceed output and read-length benchmarks set by PacBio to ensure you receive high-quality results.

Contact our team to discuss how Iso-Seq on the new PacBio system can improve your NGS projects.


What is Iso-Seq?

The Iso-Seq method generates full-length cDNA sequences and analyzes full-length transcript isoforms, which can be used in RNA sequencing (RNA-Seq) to:

  • See alternative start and end sites
  • Characterize splicing events
  • Find fusion transcripts
  • Identify allele-specific isoforms
  • Discover non-coding RNAs
  • Profile expression at isoform resolution
  • Predict full-length open read frames and more


What is the output of Iso-Seq?

The Iso-Seq method and bioinformatics workflow outputs high-quality, full-length transcript sequences of 10kb or longer. The high accuracy of high-fidelity (HiFi) reads ensure that SNPs can be identified, as well as UMIs and barcodes used for single-cell studies.

Iso-Seq delivers full-length transcript isoforms with no assembly required. Unlike short-read RNA-Seq methods, Iso-Seq method reads span the whole transcript, capturing even untranslated regions.


What is Iso-Seq analysis using PacBio technology?

Iso-Seq analysis empowers you to discover novel transcripts and genes, identify fusion genes, and annotate isoforms and alternative splicing events. Iso-Seq analysis uses highly accurate long reads (HiFi reads) generated with the CCS algorithm to cluster and generate full-length, high-quality transcript isoforms.


What is the depth of Iso-Seq?

The read depth of Iso-Seq is 1.6M full length transcripts.



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Features & Benefits

Optimized workflows deliver best output and accuracy when compared to PacBio benchmarks
Dedicated support from experienced Azenta scientists
Lower input material requirements when compared to older technologies
Cost-effective with fast turnaround times

NGS Platforms

Azenta is a qualified service provider for Illumina and PacBio NGS platforms. For information on our NGS platforms as well as recommended configurations for your projects, please visit the NGS Platforms page.



All customers receive their raw data as BAM files. Additional data analysis including: annotation and full-length non-chimeric reads are available. Other custom reports are also available on request.

How To Order

Email | Phone 1-877-436-3949, Ext. 1