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The immense diversity of B-cell receptors (BCR) and T-cell receptors (TCR), generated through recombination of the variable (V), diversity (D), and joining (J) loci, is key to the adaptive immune system. Our Immuno-Profiling service couples ultra-deep sequencing of V(D)J regions and customized bioinformatics to provide comprehensive characterization of the entire BCR or TCR repertoire. In addition, recent advances in microfluidic technology allow BCR and TCR chains to be paired at single-cell resolution.
Check out our latest webinar “Advancements to the 10x Genomics Chromium™, Single-Cell RNA-Seq System” below.
Request Quote Why GENEWIZ
Service Options
Analyze V(D)J sequences at the RNA level using bulk or single-cell approaches.
| Immuno-Profiling for bulk repertoire analysis |
Single-Cell Immuno-Profiling for physical pairing of chains |
|
| BCR sequencing (VH and VL chains) | Yes | Yes |
|
TCR Sequencing (α and β chains) |
Yes | Yes |
| Starting materials* | Total RNA Blood Cell pellet Tissue |
Cell suspension |
| V(D)J amplification method | Proprietary 5' RACE | Targeted enrichment of cDNA with cell-specified barcodes |
| Physical pairing of VH/VL or α/β chains | No | Yes |
*Visit our Sample Submissions Guidelines for more details.
Workflow
Features & Benefits
BCR and TCR analysis available for multiple species, including human and mouse
Easy-to-interpret reports enable in-depth data analysis
Flexible sample requirements facilitate project design
Ph.D.-level consultation provides guidance before, during and after the project
Industry-leading turnaround time
Comprehensive research solutions with downstream synthetic DNA libraries available
Technical Resources
Advancements to the 10x Genomics Chromium™, Single-Cell RNA-Seq System
In this webinar, we review how recent advancements of the 10x Genomics Chromium™ system, coupled with GENEWIZ developmental efforts, can benefit the researcher. In addition, we spotlight the new Single-Cell V(D)J Solution, which can be used in conjunction with single-cell transcriptome profiling to identify pairing of full-length BCR and TCR sequences.
Example Report: Immuno-Profiling
Interactive HTML reports include the following:
- CDR3 amino acid length distribution
- Gene usage analysis
- Top V(D)J combination
- Linked CDR sequences at the nucleotide and amino acid level
- CDR clustering analysis
Example Report: Single-Cell Immuno-Profiling
The LoupeTM Cell Browser from 10x Genomics provides interactive tools including the following:
- Finding significant genes
- Identifying cell types
- Exploring substructure
- Interated gene expression and V(D)J analysis
