HIGH-THROUGHPUT GENE EXPRESSION SCREENING

 

Based on next generation sequencing, Azenta Life Sciences’ high-throughput gene expression (HT-GEx) screening, similar to DRUG-Seq, is an optimal approach for drug discovery and analysis. Unlike microarrays which bear inherent limitations, this method offers rapid, unbiased 3' gene expression screening that provides a complete cell-to-data analysis of the transcriptome at a lower cost than standard RNA sequencing. Working directly from lysate, high-throughput gene expression screening saves both time and money by removing the need to purify RNA and performs 3’ transcript tagging that reduces the required sequencing depth.


   

Applications

Drug Discovery

Compound Treatment Phenotyping

CRISPR Treatment Phenotyping

Cell Response Screening

HIGH-THROUGHPUT GENE EXPRESSION SCREENING WORKFLOW

 



Technology Comparison

Metric HT-GEx Screening mRNA-Seq Total RNA-Seq Iso-Seq
 Reads/Sample  ~1-2M Reads  ~10-30M Reads ~20-45M Reads  ~1-2M Reads 
 Min. Sample Size  96/384  1  1
Cost  $ $$  $$$   $$$$
 Starting Material  Cell Lysate >20ng Total RNA   >100ng Total RNA >1ug Total RNA 
 Selection Method  Poly(A) +   Poly(A) +  rRNA -   Poly(A) +
 Detection Level  3' Tails of Genes  Poly(A) Transcript All Transcript   Full-Length Poly(A) Transcript
Gene Expression

Variant Detection

Alternative Splicing
 Transcriptome Assembly

Suitable method | Preferred method | Not a suitable method



Deliverables

 

Standard Deliverables

  • FASTQ files
  • Quality summary report

Advanced Analysis

(In addition to standard deliverables)

  • UMI extraction, mapping, counting, and transformation
  • Normalization
  • Differential gene expression and clustering by compound/treatment

Custom bioinformatics analysis and reports are available. Please contact us about how we can customize the analysis to answer your biological question.

Features & Benefits

 
Digital Spatial Profiling Stains

Fast Turnaround Times

Starting at just 2 weeks for quick target discovery

Digital Spatial Profiling Target Areas

High-Throughput Capacity

To rapidly scale up pilot or discovery projects

Photocleave Barcodes

Superior Data Quality

Exceeding manufacturers’ benchmarks

High-throughput, cell-to-data solution for compound screening

Low-cost, unbiased coverage for use in selection of drug candidates

Receive detailed expression data for each well across multiple 96- or 384-well plates

Free technical consultation and customer support from Ph.D.-level project managers and lab scientists

Webinar Series | Advancing Transcriptomics: Gene Expression Screening, Single-Cell RNA-Seq, and Beyond

With this two-part webinar series, go beyond traditional transcriptomics and learn about the various NGS approaches available for gene expression analysis. In part 1, we take an in-depth look at various gene expression approaches, including RNA-Seq, single-cell RNA-Seq, digital spatial profiling, and more. In part 2, we explore the data generated from these approaches and how they can complement each other and confirm findings.

High-Plex Spatial Analysis of Tissue Microenvironments

Tech Note | Achieving Phenotypic Profiling for Rapid Drug Discovery with High-Throughput Gene Expression Screening

High-throughput technologies are critical in performing phenotypic profiling for drug discovery applications. In this tech note, Azenta Life Sciences discusses the challenges associated with traditional approaches, such as microarrays and RNA sequencing, and offers an optimized assay to achieve high-quality phenotypic profiling at a reduced cost for rapid drug discovery.

High-Plex Spatial Analysis of Tissue Microenvironments

Information Sheet | High-Throughput Gene Expression (HT-GEx) Screening

Download our information sheet for a brief overview of Azenta Life Scences' high-throughput gene expression screening service.                                         

 

NGS Platforms

Azenta is a certified service provider for Illumina and other cutting-edge NGS platforms. For information on our NGS platforms, as well as recommended configurations of your projects, please visit the NGS Platforms page. Azenta does not guarantee data output or quality for sequencing-only projects. 



How To Order


Email | Phone 1-877-436-3949, Ext. 1